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Showing: 801 - 827 of 827 results

  2. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    APEX2-GBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67650-plasmid-map-sequence-id-116990

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  6. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Use
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  8. pAW240

    Plasmid
    #170791
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    10B2 (P1 specific promoter)
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170791-plasmid-map-sequence-id-339123

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  9. pAW212

    Plasmid
    #172196
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    10B2 (P1 specific promoter)
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172196-plasmid-map-sequence-id-338565

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  14. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid
    #157813
    Purpose
    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection
    Depositor
    Zoltan Lipinszki
    Article
    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:
    Insert
    GFP-binding protein
    Use
    For drosophila cells
    Tags
    3xFlag
    Expression
    Insect
    Mutation
    Promoter
    metallothionein
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157813-plasmid-map-sequence-id-311081

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  17. pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

    Plasmid
    #225586
    Purpose
    Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter
    Depositor
    William McEwan
    Article
    Benn et al Science. 2024 Aug 30;385(6712):1009-1016
    Insert
    F8.2(S54L+T127A)-T2A-mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    225586-plasmid-map-sequence-id-446527

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  22. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid
    #224497
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    224497-plasmid-map-sequence-id-447355

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  25. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid
    #224496
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224496-plasmid-map-sequence-id-447065

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  27. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222853
    Purpose
    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222853-plasmid-map-sequence-id-445015

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Showing: 801 - 827 of 827 results