-
Plasmid#115158PurposeVector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.DepositorInsertmW attP-CC FRT
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBRC169
Plasmid#79670PurposeDsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (169–225)/RC169ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRN168
Plasmid#79668PurposeDsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (1–168)/RN168ExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
OA-986B
Plasmid#124999PurposeExpresses dCas9-VPR driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterUbiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo
Plasmid#140166PurposeExpresses a regulatable secretory cargo for Drosophila melanogasterDepositorInsertssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSARN
Plasmid#62295Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p160Tol2-her4.3:switchCas9
Plasmid#227680PurposeExpression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafishDepositorInsertsloxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA
UseCRISPRTagsExpressionMutationPromoterU6 and her4.3Available sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
OA-986C
Plasmid#125000PurposeExpresses dCas9-VPR driven by the bottleneck promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterbottleneckAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986D
Plasmid#125001PurposeExpresses dCas9-VP64 driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VP64
UsePiggybackTagsExpressionInsectMutationPromoterubiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-986E
Plasmid#125002PurposeExpresses dCas9-VP64 driven by the bottleneck promoterDepositorInsertdCas9-VP64
UsePiggybackTagsExpressionInsectMutationPromoterbottleneckAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fSST-RFP
Plasmid#22913DepositorInsertfugu somatostatin promoter driving DsRed 2
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5
Plasmid#61255PurposeCRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIRES RED/SP M87C448Y
Plasmid#89324PurposeExpression of RFP and M87C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 deletedPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
vas-Cas9.874Z
Plasmid#112687PurposeExpress hSpCas9 under vasa promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromotervasaAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
RT3REVIR
Plasmid#111168PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseRetroviralTagsdsRed and mVenusExpressionMutationPromoterT3GAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT3REV
Plasmid#111174PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseLentiviralTagsDsRed and VenusExpressionMutationPromoterT3GAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
Plasmid#61257PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
NCVB-ssGrl1-ESCargo*
Plasmid#140167PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophilaDepositorInsertssGrl1-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1*
Plasmid#104000PurposePlasmid encoding mRFP1*DepositorInsertmRFP1*
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLAVIKEF...T…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1
Plasmid#104001PurposePlasmid encoding mRFP1DepositorInsertmRFP1
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFU-cMVIIA-PE
Plasmid#24147DepositorInsertloxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Conus magus, Aequorea victoria, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJL6
Plasmid#184134PurposeFor labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.DepositorInsertCELE_VW02B12L.1 (vha-6 Nematode)
UseTagsRFPExpressionWormMutationPromoterPvha-6 (894bp)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Egr2ASRNA
Plasmid#177737PurposeEgr2 AS-RNA lentiviral expression vectorDepositorInsertEgr2-AS-RNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
Plasmid#131618PurposeExpresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.DepositorInsertsPuroR[dsxM]
NeoR[traF]
UseTagsExpressionInsectMutationPromoterOpie2Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEED-LoxP-sfGFP:ALFA
Plasmid#219660PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.DepositorInsertsfGFP:ALFA SEED Cassette (LoxP)
UseTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only