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Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOPO iRFP670
Plasmid#68443PurposeTOPO construct expressing iRFP670 for generation of GMAP-compatible geneDepositorInsertiRFP670
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKO2
Plasmid#68442PurposeTOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible geneDepositorInsertmKusabira-Orange2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP
Plasmid#185686PurposeLow-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.DepositorInsertERmembrane-GST-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
EZ-L498
Plasmid#131771PurposePPGK1_FUSN_Citrine _PixE_TCYC1DepositorInsertPPGK1_FUSN_Citrine _PixE_TCYC1
UseIntegration into his3 locusTagsExpressionYeastMutationPromoterPGKAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TOPO Venus
Plasmid#68493PurposeTOPO construct expressing Venus for generation of GMAP-compatible geneDepositorInsertsplit Venus
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag DK
Plasmid#112635PurposeSpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DKDepositorInsertSpyTag-ELP-SpyTag DK
UseTagsHis6ExpressionBacterialMutationContains central cysteine. Reactive aspartic acid…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO CloverCP
Plasmid#68444PurposeTOPO construct expressing CloverCP for generation of GMAP-compatible geneDepositorInsertCloverCP
UseGmapTags"CP" degradation domainExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO mT2
Plasmid#68491PurposeTOPO construct expressing mT2 for generation of GMAP-compatible geneDepositorInsertmTurquoise2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSuper TR G414C
Plasmid#207612PurposeU3 promoter driven expression of the telomerase RNA with a G414C CAB box mutationDepositorInsertTelomerase RNA G414C
UseTagsExpressionMammalianMutationPromoterU1Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-T-Lg
Plasmid#216977PurposeExpression of GvpT-Linker-Large BiTDepositorInsertGvpT-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaRLS
Plasmid#216955PurposeMega' with knockout of GvpR, GvpL and GvpSDepositorInsertMega' with knockout of GvpR, GvpL and GvpS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBSU
Plasmid#216953PurposeMega' with knockout of GvpB, GvpS and GvpUDepositorInsertMega' with knockout of GvpB, GvpS and GvpU
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaLSK
Plasmid#216957PurposeMega' with knockout of GvpL, GvpS and GvpKDepositorInsertMega' with knockout of GvpL, GvpS and GvpK
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaBRG
Plasmid#216945PurposeMega' with knockout of GvpB, GvpR and GvpGDepositorInsertMega' with knockout of GvpB, GvpR and GvpG
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Sm-U
Plasmid#217003PurposeExpression of Small BiT-Linker-GvpUDepositorInsertSmall BiT-Linker-GvpU
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only