-
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZY101
Plasmid#73932PurposeThis binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.htmlDepositorInsertbar
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH51266
Plasmid#50267DepositorInsertpromoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pORF1
Plasmid#206025PurposeAn expression vector for mouse LINE1 ORF1DepositorInsertORF1 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PLux0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ4004
Plasmid#204401PurposeA 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsmCinR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PCin0102DAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; crRNA under control of PermE*Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
A0A075HNX4
Plasmid#177695Purposeexpresses a fatty alcohol oxidase in E. coliDepositorInsertLong chain alcohol oxidase
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
JP1520 PIK3CA E545K HA
Plasmid#14571DepositorInsertPIK3CA E545K (PIK3CA Bovine)
UseRetroviralTagsHA tagExpressionMammalianMutationE545KPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCI-NLS-BirA-2A-mCherry
Plasmid#127781PurposeBirA construct in a PCI nls backbone driving mCherry activityDepositorInsertBirA
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-RFP
Plasmid#97149PurposeAs a control of miRNA spongeDepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a
Plasmid#107593PurposeAn entry vector with U6a promoter driving guide RNA expressionDepositorInsertU6a promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SpSrtA
Plasmid#64987PurposeExpresses the wild type of Streptococcus pyogenes sortase A in E. coliDepositorInsertS. pyogenes sortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-81PromoterT7 promoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC90-pmax-DEST
Plasmid#48222PurposeGateway Destination vector derived from Clontech's pmax (CAGGS) expression vectorDepositorTypeEmpty backboneUseCRISPR; Gateway destinationTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pICSL70004
Plasmid#50334DepositorInsertkanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BD2
Plasmid#37486DepositorInsertloxP-PGK-Hyg-loxP
UseIpsc gene targetingTagsExpressionMutationPromoterPGKAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
UseTags6xHIS-TwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox9_enh_366_Citrine
Plasmid#130593Purposeenhancer reporterDepositorInsertSox9_enh_366
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ef1a_NANOG_Ires_Puro
Plasmid#18920DepositorInsertNANOG (NANOG Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only