Addgene

We narrowed to 35,252 results for: lat

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SARS CoV-2 HexaPro spike (bio-His)

Plasmid

#166856

Purpose

Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.

Depositor

Evelien Bunnik

Article

Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

Insert

SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)

Use

Tags

8xHis and Biotinylation site

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

Plasmid

#71828

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST Halo-flag-CAD

Plasmid

#188117

Purpose

Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag and Halo tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

Plasmid

#162020

Purpose

Expression of GEF dead IQSEC1 mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Tags

Expression

Mutation

EK

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPHAGE-CR-PINK1-C-TAP

Plasmid

#128510

Purpose

Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PTEN induced kinase 1 (PINK1 Human)

Use

Lentiviral

Tags

FLAG and HA tags

Expression

Mammalian

Mutation

Synonymous silent mutation in Q5 to render sgRNA …

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-ID2-P2A-mCherry

Plasmid

#203865

Purpose

Tet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

ID2 (ID2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-GLI1-P2A-mCherry

Plasmid

#203858

Purpose

Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

Plasmid

#172618

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Thr283Ala

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND3-Puro

Plasmid

#172619

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

Plasmid

#75082

Purpose

An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Nuc-eYFP

Use

AAV and Cre/Lox

Tags

NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-MAFB-P2A-mCherry

Plasmid

#203866

Purpose

Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

MAFB (MAFB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-Tau-nYFP

Plasmid

#92204

Purpose

Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Venus1-Y705F-STAT3

Plasmid

#123172

Purpose

Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Y705F substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

Plasmid

#172647

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDN-D2irTNG4kwh

Plasmid

#44722

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
htetR::NLS::EGFP

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

Plasmid

#69114

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

L1939A and L1942A amino acid substitutions. Sile…

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND2-Puro

Plasmid

#172626

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Venus2-WTSTAT3

Plasmid

#123165

Purpose

Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

none

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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