Addgene

We narrowed to 11,041 results for: cel.2

Showing: 4121 - 4140 of 11041 results

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pFX-Venus-KRasCT

Plasmid

#175834

Purpose

Express Venus with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Venus with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pZ004 (LseC2c2 locus)

Plasmid

#70170

Purpose

Expresses LseC2c2 locus with one spacer in array

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

LseC2c2 locus with one spacer in array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM7i1

Plasmid

#59602

Purpose

Expression of shRNA against human ADAM7

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM7 (ADAM7 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pHyg-CUbo(K48R/K63R)-yeGFP-His6

Plasmid

#212785

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-CUbo-VSV

Plasmid

#212733

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-CUbo-VSV

Plasmid

#212732

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-VSV

Plasmid

#212730

Purpose

Copper-inducible expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-VSV

Plasmid

#212729

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-VSV

Plasmid

#212728

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K63R)-GFP

Plasmid

#212722

Purpose

Bacterial expression of His6-CUbo(K63R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis-CUbo(K63R)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K48R)-GFP

Plasmid

#212721

Purpose

Bacterial expression of His6-CUbo(K48R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Use

Tags

6xHis-CUbo(K48R)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-TEV2-Ubc7-His6

Plasmid

#212713

Purpose

Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Use

Tags

6xHis and MBP-TEV2

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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eGFP-RAPH1-F4

Plasmid

#194863

Purpose

Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 1062-1250

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-RAPH1-F3

Plasmid

#194862

Purpose

Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 868-1062

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-RAPH1-F2

Plasmid

#194861

Purpose

Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 535-868

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-6xhis-sumo-CeMIGA(106-496)

Plasmid

#192832

Purpose

Express C.elegans MIGA fragment 106-496 in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Mitoguardin homologue (miga-1 Nematode)

Use

Tags

6xhis sumo

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Sirius-KRasCT

Plasmid

#175831

Purpose

Express Sirius with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-KRasCT

Plasmid

#175832

Purpose

Express SECFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-Lifeact-EGFP

Plasmid

#174477

Purpose

Express EGFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-SECFP

Plasmid

#174476

Purpose

Express SECFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with actin-targeting sequence

Use

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge