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pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

Plasmid

#194016

Purpose

Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

Depositor

Larry Young

Article

Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

Inserts

sgRNA(Oxtr.2)
eGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

cmb and u6

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

Plasmid

#196112

Purpose

mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP-EF1-2xCMV-EF1-mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196111

Purpose

mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zmiRFP2-EF1-2xCMV-EF1-mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

Plasmid

#196109

Purpose

mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zTagRFP658-EF1-2xCMV-EF1-mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3

Plasmid

#196110

Purpose

mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3

Depositor

Reinhard Köster

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

zemiRFP2-EF1-2xCMV-EF1-mClover3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta55-118-10xHis

Plasmid

#194180

Purpose

Expresses MPP8-delta55-118_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta55-118 (chromo domain deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta2-441-10xHis

Plasmid

#194181

Purpose

Expresses MPP8-delta2-441_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta2-441

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta442-860-10xHis

Plasmid

#194182

Purpose

Expresses MPP8-delta442-860_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta442-860

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta600-728-10xHis

Plasmid

#194183

Purpose

Expresses MPP8-delta600-728_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta600-728 (4xANK deletion)

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RPS15A-3xHA plasmid

Plasmid

#192245

Purpose

overexpression vector of RPS15A-3XHA

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

RPS15A CDS without stop coden (RPS15A Human)

Use

Lentiviral

Tags

3xHA

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV myc-Arrestin-TEVp

Plasmid

#173115

Purpose

Expresses arrestin receiver construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

myc-Arrestin-TEVp

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17

Plasmid

#193299

Purpose

tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Klf4-IRES-Sox2-17 (Sox2, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagC-PrP-SBP-HsNot10_AH

Plasmid

#148844

Purpose

Mammalian Expression of HsNot10

Depositor

Elisa Izaurralde

Insert

HsNot10 (CNOT10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagC-PrP-SBP-HsNot11_AH

Plasmid

#148845

Purpose

Mammalian Expression of HsNot11

Depositor

Elisa Izaurralde

Insert

HsNot11 (CNOT11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

Plasmid

#148440

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation C840Tand sequence differs in …

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-EGFP_W

Plasmid

#147820

Purpose

Mammalian Expression of EGFP

Depositor

Elisa Izaurralde

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.337T>C-Flag

Plasmid

#169169

Purpose

Expresses EPHX1 c.337T>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-Entry-EPHX1c.416A>G-Flag

Plasmid

#169170

Purpose

Expresses EPHX1 c.416A>G in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

c.337T>C

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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