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Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)
Plasmid#208959PurposeA variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)
Plasmid#208958PurposeA variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_CoV2_P1
Plasmid#170450PurposeExpress SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.DepositorInsertSARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
UseTagsNAExpressionMammalianMutationL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…PromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001149093)
Plasmid#77788Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001147355)
Plasmid#77787Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MEK1 (S218D,S222D)-pcw107
Plasmid#64604Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsExpressionMutationS218D, S222DPromoterPGKAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAP2K1_p.C121S
Plasmid#81669PurposeGateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.DepositorInsertMAP2K1 (MAP2K1 Human)
UseGateway entry vectorTagsExpressionMutationC121S; I310NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001147498)
Plasmid#77789Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-P1-like
Plasmid#173789PurposeMammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)DepositorInsertSpike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1-pcw107-V5
Plasmid#64650Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
JNK2 WT O/E (MAPK9)-pcw107-V5
Plasmid#64617Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAPK9 (transcript variant JNK2-a2) (MAPK9 Human)
UseLentiviralTagsV5ExpressionMutationnonePromoterPGKAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKK6 (S207E,T211E)-pcw107
Plasmid#64625Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertMAP2K6 (MAP2K6 Human)
UseLentiviralTagsFLAGExpressionMutationS207E,T211EPromoterPGKAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BAP1-010
Plasmid#68366Purposestable expression of BAP1 delta 221-238 in mammalian cellsDepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationdelta 221-238, splice variant found in human meso…PromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-S2P-P.1-AVI
Plasmid#176327PurposeExpresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-P.1 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAP2K1_WT
Plasmid#81920PurposeGateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.DepositorInsertMAP2K1 (MAP2K1 Human)
UseGateway entry vectorTagsExpressionMutationI310NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shGDF11 #1
Plasmid#83083PurposeLentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAP2K1_p.P124S
Plasmid#81703PurposeGateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.DepositorInsertMAP2K1 (MAP2K1 Human)
UseGateway entry vectorTagsExpressionMutationP124S; I310NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)
Plasmid#217800PurposeVariant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promotersDepositorInsertPCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only