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Plasmid#207722PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pMIAII-Cd4
Plasmid#194401PurposeRetroviral expression of mouse CD4DepositorInsertCd4 (Cd4 Mouse)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.18 CMV-Luc
Plasmid#100984PurposeCMV luciferase reporter - firefly luciferase driven by CMV promoterDepositorInsertCMV
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI118-SiT-Cas12a-[Ind]
Plasmid#128406PurposeInducible expression of both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-K560-24xGCN4_v4
Plasmid#72229PurposeA fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide arrayDepositorInsertK560 kinesin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA-lib 2.0
Plasmid#89638PurposesgRNA expression in mammalian cells after Gateway cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLOW30
Plasmid#172697PurposeC. elegans mScarlet co-injection markerDepositorInsertPmyo-2::mScarlet
UseTagsmScarletExpressionWormMutationPromotermyo-2Available sinceSept. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB506
Plasmid#74848PurposeGateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA
Plasmid#89459Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorInsertCHD7 (CHD7 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3297
Plasmid#144773PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2863
Plasmid#143646PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSREBF1 (SREBF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-zeo
Plasmid#160091PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti Dest PuroR R1-R2
Plasmid#84575PurposeGateway destination vector expressing Puromycin resistance under a PGK promoterDepositorTypeEmpty backboneUseLentiviral; GatewayTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR
Plasmid#126679PurposeFluorescent reporter for cyclin E/A-CDK and CDK4/6 activitiesDepositorInsertRb (a.a 886-928)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)
Plasmid#199165Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+36)
UseTagsMGHHHHHHGGAExpressionMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-NLS-HA-TurboID-PCNA
Plasmid#215074PurposeExpresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.DepositorInsertPCNA (PCNA Human)
UseRetroviralTagsNLS-HA-TurboIDExpressionMammalianMutationPromoterLTRAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-Mito
Plasmid#36208PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
UseTagsNanoLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hG9a
Plasmid#33024DepositorInserthG9a
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
yeast_DualReporter
Plasmid#127546PurposeA dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF, TEF2, URA3, bla (E. coli), and pTpA+MCSAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
UseTagsExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-emiRFP670
Plasmid#197200PurposeExpresses the protein of emiRFP670 in mammalian cellsDepositorInsertemiRFP670
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TGNP-N-10
Plasmid#55145PurposeLocalization: Golgi Complex, Excitation: 587, Emission: 610DepositorInsertTGNP (TGOLN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKH3
Plasmid#12555DepositorTypeEmpty backboneUseTags3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B13-WT-FLAGC
Plasmid#184501PurposeExpresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsert17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)
UseTagsFLAGC-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H2B-iRFP720
Plasmid#128961PurposeLentiviral plasmid for expression of far red iRFP720 nuclear markerDepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57BsaI
Plasmid#128859PurposeModule cloning vectorDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TRE3G-CMV-P
Plasmid#192884PurposeCloning for Tet regulatable expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDCC6
Plasmid#59985PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassetteDepositorInsertsCas9
U6-2>sgRNA
UseCRISPRTags3xFLAGExpressionMutationPromoterU6-96Ab and hsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas ServiceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Cx43-7
Plasmid#58009PurposeLocalization: Gap Junctions, Excitation: 555, Emission: 584DepositorInsertCx43
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-GFPd2
Plasmid#14760DepositorInsertEGFPd2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-mSc
Plasmid#187842PurposeConstitutive mScarlet-I-expression vector for Fusobacterium nucleatumDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRN111
Plasmid#84463PurposepJB38-NWMN29-30 + SarA_P1-mCherry-TermDepositorInsertSarA_P1-mCherry from pRN11
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Golgi-7
Plasmid#58100PurposeLocalization: Golgi, Excitation: 554, Emission: 581DepositorInsertGolgi (B4GALT1 Human)
UseTagstdTomatoExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3418
Plasmid#144894PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFE2L2 (NFE2L2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMSCV-IRES-DsRed FP (pMIDsRed II)
Plasmid#52110Purposea bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-DsRedExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Axon-jGCaMP8s
Plasmid#172720PurposeAAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.DepositorInsertAxon-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1c
Plasmid#131813PurposeExpresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationThis is the cytoplasmic mutant causing AML, where…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recX-sacB
Plasmid#158718PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.DepositorInsertrecX
UseTagsExpressionBacterialMutationPromoterPmycAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHP306 - Ub - no Lys
Plasmid#32150DepositorUseTagsExpressionYeastMutationNo Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG
Plasmid#158127PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertmTagBFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-DddA11
Plasmid#179681PurposeExpression of base editor in mammalian cellsDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR
UseTagsExpressionMammalianMutationS1330I A1341V N1342S E1370K T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL2-mGold-P2A-EBFP2
Plasmid#157998PurposeCo-expression of mGold and EBFP2 in mammalian cells.DepositorInsertmGold-P2A-EBFP2
UseTagsExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only