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pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2384
Plasmid#142411PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMBD3 (MBD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-mFzd5
Plasmid#102867PurposeExpresses mouse Fzd5 in mammalian cellsDepositorInsertFrizzled 5 (Fzd5 Mouse)
UseLentiviralTagsExpressionMammalianMutationA to G substitution at nucleotide 699 of Fzd5 ORF…PromoterEF1AAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0793
Plasmid#142692PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF5 (ATF5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-TAZ
Plasmid#174176PurposeLentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.DepositorInsertTAZ (WWTR1 Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWRS_1001
Plasmid#192205Purposelentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAsDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and RNAiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
BirA*-HA-NES
Plasmid#214473PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and NES (Nuclear Exclusion Sequence)ExpressionMammalianMutationR118GPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-P2A-EGFP
Plasmid#188898PurposeHuman lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9
UseLentiviralTagsHA-2xNLS and P2A-GFPExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOneZeo-ORF18(WT)-3xFLAG
Plasmid#129746PurposeDox inducible expression of KSHV ORF18-3xFLAG in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterTRE3Gs and Tet inducible promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3B
Plasmid#175753PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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TFORF2530
Plasmid#144035PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX8 (SOX8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-Flag-h-SNRNP40
Plasmid#134254PurposeLentivector encoding Flag-tagged human SNRNP40DepositorInsertSNRP40 (SNRNP40 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-Nanog-EGFP
Plasmid#21321PurposeLentiviral vector, expresses EGFP under Nanog promoterDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterNanog promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationRemoved stop codon to fuse it to the 2A-eGFPnls i…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-Sec23B
Plasmid#66598PurposeExpresses Sec23B as a fusion with mGFPDepositorInsertSec23B (SEC23B Human)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCD71-2
Plasmid#46918PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK1-K179E
Plasmid#199644PurposeExpresses kinase-inactive VRK1 (K179E)DepositorInsertVRK1 (VRK1 Human)
UseLentiviralTagsExpressionMutationK179EPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gRNA-puro
Plasmid#180426PurposeExpresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.DepositorInsertgRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only
