Addgene

We narrowed to 13,534 results for: pET

Showing: 4101 - 4120 of 13534 results

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pCri-11b

Plasmid

#61333

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only
K406

Plasmid

#129764

Purpose

Expresses Drosophila Kinesin-1 truncated after neck-coil at 406

Depositor

William Hancock

Insert

KHC (Khc Fly)

Use

Tags

His6

Expression

Bacterial

Mutation

Truncation at 406

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Use

Tags

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
pUG-301

Plasmid

#104730

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC10_EL3

Use

Tags

HIS-Ubq, HA-E2

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPcCas12a

Plasmid

#174681

Purpose

Expresses 6xHis-MBP tagged PcCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPcCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huCMtCas12a

Plasmid

#174680

Purpose

Expresses 6xHis-MBP tagged CMtCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huCMtCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPdCas12a

Plasmid

#174682

Purpose

Expresses 6xHis-MBP tagged PdCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPdCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCri-4a

Plasmid

#61314

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and TRX-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-7b

Plasmid

#61330

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-6a

Plasmid

#61315

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

GST-tag and His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-9a-Strep

Plasmid

#61341

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

Strep-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-1a

Plasmid

#60816

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and MBP-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-14a

Plasmid

#61322

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and LSL-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-7a

Plasmid

#61316

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAM303

Plasmid

#226136

Purpose

Expression of His6-GG-UbK48R

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GG-UbK48R

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM294

Plasmid

#226130

Purpose

Expression of Ufd1-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1-3C-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM292

Plasmid

#226129

Purpose

Expression of His6-SUMO-Ub-mEos3.2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-intein-CBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM296

Plasmid

#226132

Purpose

Expression of Ufd1L55R-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1L55R-3C-His6

Use

Tags

Expression

Bacterial

Mutation

L55R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM301

Plasmid

#226134

Purpose

Expression of MC-Ub-SH3-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-SH3-3C-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge