Showing: 4101 - 4120 of 5864 results
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Plasmid#110166PurposeminiMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43A, T55A, T62A.…Promoterlin-31 promoter/enhancerAvailabilityAcademic Institutions and Nonprofits only -
pCC336
Plasmid#110167PurposeminiMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43E, T55E, T62E.…Promoterlin-31 promoter/enhancerAvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Target Zfp462-Klf4SE
Plasmid#127668PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Promoter Only Target
Plasmid#127669PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II tension sensor (F40-based)
Plasmid#118715PurposeThe F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II no force control (F40-based)
Plasmid#118716PurposeThe no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationtruncation after aa1353PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II tension sensor (FL-based)
Plasmid#118721PurposeThe FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II no force control (FL-based)
Plasmid#118724PurposeThe no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationtruncation after aa1353PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#118725PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#118726PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationtruncation after aa1952PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pVC-FUS-R521C
Plasmid#175067Purposeexpresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cellsDepositorInsertFUS glycine rich protein (FUS Human)
UseTagsHA and mVenus + mCherryExpressionMammalianMutationchanged Arginin 521 to Cystein; mVenus: C-Termina…PromoterCMV PromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLQ10878
Plasmid#183964PurposeCAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD156R, E292R, D235R, D350RPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pT22UbGfpnE4PCh(#301)
Plasmid#184066Purposecyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenicDepositorInserteGFP-nls-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pT22Ubm4Eng2bKRPC(#493)
Plasmid#184091Purposecyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenicDepositorInsert4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags4xMycExpressionMutationZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pT22gcfUbm5En25EE4PCh(#552)
Plasmid#184092Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(5E)-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pJZC533
Plasmid#135435PurposeYeast integrating plasmid for pTEF1-mCherry reporter geneDepositorInsertmCherry
UseTagsExpressionYeastMutationcodon optimized for S. cerevisiaePromoterpTEF1AvailabilityAcademic Institutions and Nonprofits only -
pMJB1
Plasmid#160429PurposeYeast vector for expressing mCherry tagged genes under the GAL1 promoterDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
phlFs-mLin
Plasmid#140792PurposeCMV-phlFo-phlFR-2A-mCherry mammalian synthetic gene circuitDepositorInsertphlFs-mLinearizer
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
phlFd-mLin
Plasmid#140793PurposeCMV-phlFo2-phlFR-2A-mCherry mammalian synthetic gene circuitDepositorInsertphlFd-mLinearizer
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pVW296
Plasmid#139496PurposeExpression vector for the PP7-mCherry protein in S. cerevisiaeDepositorInsertpADH1 PP7∆FG-mCherry tCYC1
UseTagsExpressionYeastMutation∆FGPromoterpADH1AvailabilityAcademic Institutions and Nonprofits only
Showing: 4101 - 4120 of 5864 results