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Showing: 4101 - 4120 of 22178 results

  1. pB2H12

    Plasmid
    #163837
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTPN12 E57D
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163837-plasmid-map-sequence-id-337607

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  7. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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  9. TOPO mMecp2-NLucTom

    Plasmid
    #159282
    Purpose
    To tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.
    Depositor
    Joost Gribnau
    Article
    Mira-Bontenbal et al Stem Cell Reports. 2022 Jan 29. pii: S22
    Insert
    Mecp2 (Mecp2 Mouse)
    Use
    Tags
    Nluciferase-P2A-TdTomato
    Expression
    Mammalian
    Mutation
    Nluciferase-TdTomato fusion
    Promoter
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    159282-plasmid-map-sequence-id-313970

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  12. pEGFP-N1-HSD17B11-S172L-FLAGC

    Plasmid
    #161904
    Purpose
    Expresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.
    Depositor
    Sébastien Britton
    Article
    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:
    Insert
    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    Use
    Tags
    FLAG-GFP
    Expression
    Mammalian
    Mutation
    S172L; catalytically dead mutant
    Promoter
    CMV
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161904-plasmid-map-sequence-id-320523

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Showing: 4101 - 4120 of 22178 results