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Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huErCas12a
Plasmid#174685PurposeExpresses 6xHis-MBP tagged ErCas12aDepositorArticleInserthuErCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHisExpressionBacterialMutationQ926R mutation is observed as indicated from the …PromoterT7Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorInsertcsgA (csgA )
UseTags3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…ExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-11 (AA100-373) (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
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NLS-Halo
Plasmid#200197PurposeExpression for SV40 NLSDepositorInsert3X SV40 NLS-Halo
UseTagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPTM p62 PB1 ZZ (1-167)
Plasmid#210429PurposeBacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage siteDepositorInsertp62 PB1 ZZ (1-167) (NUP62 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
UseTags6HisExpressionBacterialMutationD16A H588APromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CLAPon
Plasmid#213405PurposePeroxidase (APEX) reporter for TEV protease cleavageDepositorInsertE5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Talin Head
Plasmid#186140PurposeFor expression of recombinant Talin head domain in bacteriaDepositorInsertTLN1 (TLN1 Human)
UseTagsHis6 (tev cleavage)ExpressionBacterialMutationPromoterLacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
UseTagsExpressionBacterialMutationPromoterProD, nTETO, and pBAD /AraCAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcC)
Plasmid#176197PurposegfcC protein expressionDepositorInsertgfcC
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0281
Plasmid#162302PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, [S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC-PTP-NEO
Plasmid#51710Purposecontains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)ExpressionMutationPromoterAvailable sinceMarch 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcB)
Plasmid#176196PurposegfcB protein expressionDepositorInsertgfcB
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp1
Plasmid#167256PurposeBacterial expression of Nsp1 residues 13-127DepositorInsertNsp1 (residues 13-127) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits