Addgene

We narrowed to 15,711 results for: puromycin

Showing: 4081 - 4100 of 15711 results

Results per page:

Sort results by:

pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

Plasmid

#130683

Purpose

Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_E452del, E653_G1960del

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF704

Plasmid

#121658

Purpose

U6-sgRNA EFS-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF712

Plasmid

#121663

Purpose

U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9(C)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Fuw CBX8 quad

Plasmid

#121118

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A,R20A, R22A, W32A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R19A, R20A, R22A, W32A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-FHH-MAPKAP1RBD

Plasmid

#120710

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0+N497A+Q695A+Q926A

Plasmid

#108300

Purpose

pX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0-eSpCas9(1.1)+K855A

Plasmid

#108298

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0-eSpCas9(1.1)+K810A

Plasmid

#108297

Purpose

pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTICRISPR sgNFS1

Plasmid

#102979

Purpose

Cutting NFS1 genomic locus

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

sgNFS1 (NFS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode49

Plasmid

#83726

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode49

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode42

Plasmid

#83719

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode42

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode18

Plasmid

#83695

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode18

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode92

Plasmid

#83769

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode92

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode90

Plasmid

#83767

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode90

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode28

Plasmid

#83705

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode28

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode15

Plasmid

#83692

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode15

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-6NBC-GFP-PGK-Puro-Barcode94

Plasmid

#83771

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode94

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge