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Plasmid#202416PurposeExpression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutationDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationLacking DTHL, and H477/R479/S481>AAA mutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXSN p200 CUX1
Plasmid#90470PurposeRetroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectivelyDepositorInsertCUX1 (CUX1 Human)
UseRetroviralTagsHa and mycExpressionMammalianMutationPromoterMoloney murine leukemia virus long terminal repeatAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdICAM1-ST3-His
Plasmid#210666PurposeExpression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-BRAF-REG-Halo
Plasmid#194771PurposeMammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.DepositorInsertBRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV51Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mFgf21pro2-luc
Plasmid#101798PurposeReporter for mouse Fgf21 promoter activityDepositorInsertFibroblast growth factor 21 promoter (Fgf21 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdCD86-ST3-His
Plasmid#210664PurposeExpression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)DepositorInsertExtracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)
UseTagsHistag and Spytag003ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-KDM2B-P2A-mCherry
Plasmid#203852PurposeTet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertKDM2B (KDM2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MKK1
Plasmid#107973Purposeexpression of MAP Kinase KinaseDepositorInsertMKK1 (MAP2K1 Human)
UseTagsExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL FKBP 4K>R
Plasmid#202421PurposeExpression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsFKBP and GFPExpressionMutationK431/525/526/547RPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus1-K685R-STAT3
Plasmid#123170PurposeExpresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationK685R substitutionPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-2xFLAG-2xSTREP-CCND3-Puro
Plasmid#172622PurposeRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseRetroviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-CMV-nAChRb2(E61C)-IRES-GFP
Plasmid#164779Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationE61CPromoterCMVAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus1-Y705F/S727A-STAT3
Plasmid#123176PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus2-DeltaCT-STAT3
Plasmid#123179PurposeExpresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
UseTagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDeletion of the SH2 and TAD domains (C-terminus, …PromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGL3-mFgf21pro3-luc
Plasmid#101799PurposeReporter for mouse Fgf21 promoter activityDepositorInsertFibroblast growth factor 21 promoter (Fgf21 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD E1368A
Plasmid#188141PurposeExpresses C-terminal flag-tagged human CAD E1368A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGR3-P2A-mCherry
Plasmid#203861PurposeTet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertEGR3 (EGR3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1 Delta RC
Plasmid#164522PurposeGateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)DepositorInsertNSP1 Delta RC (ORF1ab )
UseTagsExpressionMammalianMutationInsert of NSP1 gene's CDS from SARS-CoV-2 is…PromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1396A
Plasmid#188126PurposeExpresses C-terminal flag-tagged human CAD S1396A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only