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Plasmid#27630DepositorInsertmouse myc ULK1 K46I (Myc Mouse)
UseTagsmycExpressionMammalianMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2BmGreenLantern
Plasmid#177332PurposeTo express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.DepositorInsertH2B (Hist1h2bq Rat)
UseAAVTagsmGreenLanternExpressionMammalianMutationThis fusion protein was optimized to the Human co…PromoterCAGAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR3c-V5/HIS
Plasmid#201108Purposeexpression of human FGFR3 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IGF1R-V5/HIS
Plasmid#201979Purposeexpression of human IGF1R receptor tyrosine kinase in mammalian cellsDepositorInsertIGF1R receptor tyrosine kinase (IGF1R Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR4-V5/HIS
Plasmid#201109Purposeexpression of human FGFR4 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-Gephyrin P1
Plasmid#68815Purposeexpression of fluorescent tagged Geph in mammalian cellsDepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyri…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sFlt1-i13 V5
Plasmid#86044Purposehuman Soluble Flt1 isoform i13 (terminates in intron 13)DepositorInserthuman soluble Flt1 isoform i13 (FLT1 Human)
UseTags6xHis and V5ExpressionMammalianMutationisoform i13 (terminates in intron 13)PromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AXL-V5/HIS
Plasmid#200117PurposeExpression of human AXL receptor tyrosine kinase in mammalian cellsDepositorInserthuman AXL receptor tyrosine kinase (AXL Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
sFlt1-e15a V5
Plasmid#86045Purposehuman Soluble Flt1 isoform e15a (terminates in exon 15a)DepositorInserthuman soluble Flt1 isoform e15a (FLT1 Human)
UseTags6xHis and V5ExpressionMammalianMutationisoform e15a (terminates in exon 15a)PromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk12_Nterm_FlagHa
Plasmid#127179PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertN-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UsePiggybac transposase vectorTagsFlag- HA- Tandem EpitopesExpressionMammalianMutationPromoterTetO PromoterAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMARK3 (MARK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorInsertTGFB2 (TGFB2 Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
natMX6-ins5
Plasmid#195042PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertNatR
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-R1441C
Plasmid#25046DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationArginine 1441 mutated to CysteinePromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pS6-TGFB2-H8
Plasmid#128502PurposeExpresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.DepositorInsertTGFB2 (L21-S414) (TGFB2 Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK3 K261R
Plasmid#80875Purposemammalian expression of ALK3 K261RDepositorInsertALK3 (BMPR1A Human)
UseTagsHAExpressionMammalianMutationK261R (Kinase inactive)PromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin P1
Plasmid#68816PurposeFlag tagged Geph mammalian expressionDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyrin…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MET-V5/HIS
Plasmid#201982Purposeexpression of human MET receptor tyrosine kinase in mammalian cellsDepositorInsertMET receptor tyrosine kinase (MET Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only