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Plasmid#63598PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-RTA
Plasmid#99675PurposeExpresses dSp Cas9 fused at C term. To VP64 and RTA activation domainsDepositorArticleInsertdCas9
UseTagsVP64-RTAExpressionMammalianMutationdead Cas9PromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1820_pCR8-DR-RG6-SA-DR
Plasmid#118654PurposeTransient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway DonorDepositorInsertDR-RG6-SA-DR
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x7
Plasmid#63601PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_VPR_EYFP
Plasmid#167890PurposePiggyBac compatible plasmid expressing Cas9-VPRDepositorInsertCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEC578
Plasmid#174366PurposedCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.DepositorInsertdCas9 fused to SYNZIP18
UseTagsExpressionBacterialMutationPromoterJ23150Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (insertion 5)
Plasmid#128116PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)DepositorInsertCascAID (insertion 5)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (N39A)
Plasmid#128117PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (N39A)DepositorInsertCascAID (N39A)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS234
Plasmid#73682PurposeSapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable markerDepositorInsertGFP + Cbr-unc-119
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#113234PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATCDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CCATCPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SIX4_iso1
Plasmid#128238PurposeDonor vector for 3' FLAG tag of human SIX4_iso1DepositorInsertSIX4_iso1 Homology arms
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA1
Plasmid#170088Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
VKG1-gRNA-pX330
Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
OA-1050S (UASt-dCasRx)
Plasmid#132419PurposeExpress dCasRx under UASt promoterDepositorInsertsCasRx
UASt+hsp70
UseTagsExpressionInsectMutationPromoterUAStAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (wtAcrIIA4)
Plasmid#128115PurposeExpresses SpyCas9 fused to wild-type AcrIIA4DepositorInsertCascAID (wtAcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBC2102-reci
Plasmid#117171PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLSDepositorInsertreci_wtCas9 without NLS
UseLentiviralTagsExpressionMutationPromoterU6 PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-1
Plasmid#164858PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-1
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH184
Plasmid#122857PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (D14A/G38A)
Plasmid#128118PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)DepositorInsertCascAID (D14A/G38A)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only