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Plasmid#186887PurposeExpression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain fusion (Adamts1 Synthetic, Human)
UseLentiviralTagsExpressionMutationN-terminal truncation, MAVS transmembrane domain …PromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-orangutan cGASdeltaN-OMM
Plasmid#186888PurposeExpression of orangutan cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal MAVS transmembrane domain fusion (CGAS Synthetic, Pongo abelii)
UseLentiviralTagsExpressionMutationN-terminal truncation, MAVS transmembrane domain …PromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA
Plasmid#222861PurposeThis plasmid codes for the OgeuIscB nickaseDepositorInsertOgeuIscB Nickase
UseCRISPRTags3X HA and Nucleoplasmin NLS and ITR2ExpressionMammalianMutationE193A for nickase mutationPromoterCMVAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: NES-FKBP-iLID-LRP6c(△1-64)
Plasmid#221775PurposeExpresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cellsDepositorInsertpCMV:: NES-FKBP-iLID-LRP6c
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Loxp-CMV-Loxp-GFP-LA
Plasmid#206199PurposeExpresses GFP-lamin-A only in non-myocyte when delivered into mice carrying a cardiomyocyte-specific Myh6-Cre transgeneDepositorInsertLmna (LMNA Human)
UseAAVTagsGFPExpressionMutationPromoterCMVAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: NES-FKBP-iLID-LRP6c
Plasmid#221774PurposeExpresses NES-FKBP-iLID-LRP6c component in mammalian cellsDepositorInsertpCMV:: NES-FKBP-iLID-LRP6c
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Hbb
Plasmid#99693PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PE2-IRES-ZeoR
Plasmid#207352PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.DepositorInsertPrime editor 2 (PE2) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA
Plasmid#216319PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Cerulean + splice acceptor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA
Plasmid#216318PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertsplit cerulean fluorescent protein + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DNAJC5-ΔC30 (Δ169-198)
Plasmid#205722PurposeMammalian expression of DNAJC5-ΔC30 (Δ169-198)DepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsExpressionMammalianMutationΔ169-198PromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plx208-CMV-SspB-EGFP-VP16
Plasmid#213532PurposeExpresses one of the split transcription factor component in mammalian cellsDepositorInsertSspB-EGFP-VP16
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)
Plasmid#214277PurposeMammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFRDepositorInsertmScarlet-I-eDHFR-ORP5(253-879) (OSBPL5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'-BirA*-FLAG minimal CMV pCDNA5
Plasmid#212047PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA M. mulatta (rhesus macaque), Human, Mouse)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA*-FLAG minimal CMV pCDNA5
Plasmid#212046PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG GFP minimal CMV pCDNA5
Plasmid#212086PurposeLR vector for integration of GFP into N2a FRT rtTA3 expression cellsDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-XPACK-DNAJC5 (L115R)- HaloTag
Plasmid#205712PurposeMammalian expression of XPACK-DNAJC5 (L115R)-HaloTagDepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsHaloTag and XPACK (GCINSKRKD)ExpressionMammalianMutationL115RPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DEAD XPACK-DNAJC5 (L115R)
Plasmid#205718PurposeMammalian expression of DEAD XPACK-DNAJC5 (L115R)DepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsDead XPACK (GCINIKRKD)ExpressionMammalianMutationL115RPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only