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Showing: 4081 - 4100 of 148509 results

  1. pN1-TOMM20-mNG

    Plasmid
    #129347
    Purpose
    Mitochondrial outer membrane localization
    Depositor
    Yasushi Okada
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129347-plasmid-map-sequence-id-260190

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  2. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50580-plasmid-map-sequence-id-75637

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  3. pCW57-GFP-2A-Cas9

    Plasmid
    #170805
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide
    Depositor
    Morten Frodin
    Article
    Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170805-plasmid-map-sequence-id-338370

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  4. Mut2-RS

    Plasmid
    #182882
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut2-RS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182882-plasmid-map-sequence-id-366061

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  6. pcDNA3 NT3

    Plasmid
    #39981
    Depositor
    Yves-Alain Barde
    Insert
    Neurotrophin 3 (Ntf3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. pKL2013

    Plasmid
    #172182
    Purpose
    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.
    Depositor
    Kan Wang
    Article
    McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d
    Insert
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    172182-plasmid-map-sequence-id-339004

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  9. TFORF2793

    Plasmid
    #143619
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF282 (ZNF282 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143619-plasmid-map-sequence-id-400454

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  13. pLenti-EB1-EGFP

    Plasmid
    #118084
    Purpose
    Expresses EB1-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human EB1 (MAPRE1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118084-plasmid-map-sequence-id-230385

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  14. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  16. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  18. pETcon(-)

    Plasmid
    #41522
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Tags
    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    41522-plasmid-map-sequence-id-59598

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  19. pLV-BDNF-pHluorin

    Plasmid
    #187193
    Purpose
    To express the mouse BDNF tagged with the FP pHluorin
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF (Bdnf Mouse)
    Use
    Lentiviral
    Tags
    pPHluorin
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    187193-plasmid-map-sequence-id-373713

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  20. pGJJ045

    Plasmid
    #183621
    Purpose
    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183621-plasmid-map-sequence-id-361478

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Showing: 4081 - 4100 of 148509 results