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Plasmid#142770PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1258
Plasmid#142769PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1257
Plasmid#142768PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1256
Plasmid#142767PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1255
Plasmid#142766PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1254
Plasmid#142765PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0047
Plasmid#141492PurposeLentiviral vector for overexpressing the MAX transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAX (MAX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Td-CBEmax
Plasmid#196600PurposeMammalian expression of Td-CBEmaxDepositorInsertbpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
UseTags6*HisExpressionMammalianMutationTadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)PromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1-HiBiT
Plasmid#198310PurposeLoads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains PnfDepositorInsertPdp1-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CDKN1A_WT
Plasmid#82201PurposeGateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.DepositorInsertCDKN1A (CDKN1A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Halo-Cyto
Plasmid#124314PurposeExpresses cytosolic specific HaloTag proteinDepositorInsertHalo tag
UseTagsCytoplasm specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-HYG-YR
Plasmid#61765PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).DepositorInsertsHygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC52A1
Plasmid#132169PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC52A1 (SLC52A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-PPP1CB-miRFP703
Plasmid#178524PurposeExpresses PP1c-miRFP703 in mammalian cellsDepositorInsertPP1c-miRFP703 (PPP1CB Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AcpP pet29a C-terminal His Tag
Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorInsertAcpP (acpP E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
Plasmid#108730PurposeExpresses aa271 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertNEK7 (NEK7 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only