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Plasmid#40404DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867-2141PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus N-His/GST/LRRK2 1867-2161
Plasmid#40405DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationWT aa1867-2161PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
GMIP
Plasmid#32046PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGMIP (GMIP Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 80-357 onlyPromoterAvailable sinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIC.B3.CT0478
Plasmid#11526DepositorInsertScpB
UseTagsHisTag, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pMSP2N2
Plasmid#29520DepositorInsertMSP2N2
UseTags7-His tagExpressionBacterialMutationtandem MSP; His-tag on N-terminus followed by spa…PromoterAvailable sinceMay 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
PRDX6A-c001
Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor CommentsPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA
Plasmid#74646PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1BA (KDM5B Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationLeu101-(Gly)4-Ala374 deletion mutantPromoterPHAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI3
Plasmid#42527DepositorInsertWIPI3 (WDR45B Human)
UseTagsGST-TEVExpressionMammalianMutationgene sequence is codon optimized for expression i…PromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-Wt-NmeCas9
Plasmid#71474Purposebacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-WT-NmeCas9
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Halo-Sox13
Plasmid#200194PurposeExpression of full length Sox13DepositorInsertHalo-Sox13 (Sox13 Mouse)
UseTagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
UseTagsHis-NusA-TevExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-mCh-FYVE
Plasmid#192289PurposeExpresses GST- and mCherry-tagged HRS FYVE in e ColiDepositorInsertHRS FYVE domain
UseTagsGST-TEV-mCherryExpressionBacterialMutationPromotertacAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM468-EGFP
Plasmid#108434PurposeORBIT integrating plasmid for C-terminal tagging with EGFP.DepositorInsertattB-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-H-Lock
Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only