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pNH-TrxT
Plasmid#26106PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
psfaRb-Tn5
Plasmid#183638PurposeExpresses anti-rabbit nanobody fused with Tn5DepositorInsertanti rabbit secondary nanobody fused to Tn5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PIA900
Plasmid#104401PurposePolycistronic vector for expression of E. coli core RNAPDepositorInsertrpoA, B, C, and Z
UseE. coli expression vectorTagsencodes a C-terminally His-tagged rpoC which can …ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUG-124
Plasmid#104635PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
UseTagsHIS-Ubq, HA-E2, GST-E3ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CTHF
Plasmid#26105PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsTEV - His6 - FlagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTSA-C
Plasmid#17329DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB
Plasmid#26107PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis6 - Z-basic - TEVExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
K560GFP
Plasmid#129761PurposeExpresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assaysDepositorInsertKHC (Khc Fly)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
KRigor980
Plasmid#129763PurposeExpresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfacesDepositorInsertKHC (Khc Fly)
UseTagsHis6ExpressionBacterialMutationR210APromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLysscys255
Plasmid#36429DepositorInsertSaporin-3/cys255
UseTagsExpressionBacterialMutationcys added at position 255PromoterT7Available sinceMay 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huTsCas12a
Plasmid#174687PurposeExpresses 6xHis-MBP tagged TsCas12aDepositorArticleInserthuTsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHAExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
