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lentiCRISPR-v2-HygR-EGFP

Plasmid

#167188

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1-GFP (27)

Plasmid

#26061

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pBI-GFP-Bub1-wt

Plasmid

#16624

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 (BUB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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STOP-eGFP-ROSA26TV

Plasmid

#11739

Depositor

Klaus Rajewsky

Article

Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

Insert

STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Tags

Expression

Mutation

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLEGFP-S727A-STAT3

Plasmid

#71446

Purpose

Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

S727A

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-eGFP

Plasmid

#193022

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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TOM20*-SBP-GFP

Plasmid

#120173

Purpose

Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)

Use

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Truncated TOM20: 1-30 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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OCT4-eGFP-2A-Puro

Plasmid

#31939

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Inserts

OCT4 (POU5F1 Human)
eGFP (eGFP )

Use

Targeting donor

Tags

eGFP

Expression

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pHR_TRE_Ncad-P2A-GFPlig

Plasmid

#133806

Purpose

inducible expression of Ncad and membrane-tethered GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

cadherin 2 (CDH2) (Cdh2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMXsIP GFP-VPS33A

Plasmid

#67022

Purpose

human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

Plasmid

#32627

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Donor + Sce BFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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mito-CIBN-GFP

Plasmid

#89480

Purpose

Encodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

mito-CIBN-GFP (CIB1 Human, Mustard Weed)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Beta1-GFP in pHcgreen

Plasmid

#69804

Purpose

Expresses Beta1-GFP integrin for normal cell transfection

Depositor

Martin Humphries

Article

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1A integrin (ITGB1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-wt

Plasmid

#12980

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pF141 pAcGFP1 SOD1WT

Plasmid

#26402

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2b wt

Plasmid

#45447

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2B (Grin2b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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Tcf/Lef:H2B-GFP

Plasmid

#32610

Depositor

Anna-Katerina Hadjantonakis

Article

Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

Insert

Tcf/Lef:H2B-GFP

Use

Tags

Expression

Mutation

Promoter

hsp68

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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TS245_pNKI-2-13_EGFP-DPN7

Plasmid

#200719

Purpose

producing Methyl Tracer EGFP protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

eGFP-DPN7

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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