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Plasmid#182654PurposeExpresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertwild type mouse neurofilament light chain (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_UQCRC2
Plasmid#177982Purposelentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2DepositorInsertsgRNA targeting UQCRC2 (UQCRC2 Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2
Plasmid#176252PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by fullDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-mKate2
Plasmid#174178PurposeExpress mKate2 with mitochondria-targeting sequence in mammalian cellsDepositorInsertmKate2 with mitochondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorInsertADAR2 (ADARB1 Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG010 (DRT type 2)
Plasmid#157888PurposeExpresses the DRT type 2 defense systemDepositorInsertDRT type 2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNAy
Plasmid#168294PurposeExpresses gRNA targeting the yellow gene in DrosophilaDepositorInsertU6.3-gRNA[y]-scaffold
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X7
Plasmid#182366PurposeExpresses Neq2X7 DNA polymeraseDepositorInsertNeq2X7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-Rab7a-7
Plasmid#56567PurposeLocalization: Ras GTPase, Excitation: 516, Emission: 529DepositorInsertRab7a (RAB7A Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCitrine-Peroxisomes-2
Plasmid#54672PurposeLocalization: Peroxisomes, Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
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MaLionG
Plasmid#113906PurposeGreen ATP sensorDepositorInsertMaLionG
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET15b/GgVcl 884-1066-ECFP
Plasmid#46182DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
VP16-hAR
Plasmid#89092Purposemammalian expression of full-length human androgen receptor with added N-terminal VP16 activation domainDepositorInserthAR (AR Human)
UseTagsVP16ExpressionMammalianMutationPromoterSV40Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-RasAR-NES
Plasmid#205567PurposeGenetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.DepositorInsertRasAR-NES (RAF1 Human, Synthetic)
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only