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Showing: 4041 - 4060 of 35252 results
  1. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid
    #69116
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 682-688 (region containin…
    Promoter
    CMV
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

    Plasmid
    #209787
    Purpose
    Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Insert
    TNT4, TadA8e, SpRY N, inteinN, miR122 TS
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    TNT4
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4041 - 4060 of 35252 results