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Showing: 4021 - 4040 of 20444 results

  11. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196986-plasmid-map-sequence-id-396953

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  15. pJAT56

    Plasmid
    #204300
    Purpose
    FExpress phiC31 integrase under heat shock control. Insert marked with 3XP3-EGFP, which expresses in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204300-plasmid-map-sequence-id-405836

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  19. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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Showing: 4021 - 4040 of 20444 results