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pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 CMV NAPA-N BGH

Plasmid

#92278

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_SCP1_CMV

Plasmid

#99314

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV TIP60 390-513

Plasmid

#78797

Purpose

To overexpress TIP60 390-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

aa390-513

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-1st-PDZ-mut

Plasmid

#27416

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
p2xFlag CMV2-YAP1-1st WW mutant

Plasmid

#19004

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
p7134 pHAGE-P-CMVt-N-HA-GAW-WDR92

Plasmid

#100161

Purpose

Expresses WDR92

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

WDR92 (WDR92 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

Plasmid

#130688

Purpose

Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

Plasmid

#130681

Purpose

Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_V2005delinsX

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4-Flag-mIL-17RA/CFP.Hygro

Plasmid

#46861

Purpose

encodes Flag-tagged mouse IL-17RA fused to Cyan Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-CFP

Use

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3

Plasmid

#62169

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Luciferase

Use

Luciferase; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV Lex VP16 (F442A) HA (P#1710)

Plasmid

#14595

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pLU-CMV-Flag-hSesn2 R419A

Plasmid

#111792

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R419A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

Plasmid

#58414

Purpose

Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA internal

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine; The sequenc…

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
STARR-seq luciferase validation vector_mP_CMV

Plasmid

#99313

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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p7139 pHAGE-P-CMVt-N-HA-GAW-PHKG2

Plasmid

#100159

Purpose

Expresses PHKG2

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

PHKG2 (PHKG2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p7138 pHAGE-P-CMVt-N-HA-GAW-PHKB

Plasmid

#100158

Purpose

Expresses PHKB

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

PHKB (PHKB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only