Addgene

We narrowed to 4,157 results for: 027

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pDONR223_PPP6C_WT

Plasmid

#81811

Purpose

Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP6C (PPP6C Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

Plasmid

#208955

Purpose

A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

Plasmid

#139447

Purpose

Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)

Plasmid

#139443

Purpose

Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

Plasmid

#139993

Purpose

CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…

Promoter

CMV and T7

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-cDAXX-0SB

Plasmid

#169728

Purpose

Mammalian expression construct of GFP tagged cDAXX-0SB

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

cDAXX-0SB (DAXX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Comprises residues 495-741 and has the major SB m…

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-eGFP

Plasmid

#122033

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-NCOA7g1 (BB34)

Plasmid

#139452

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

Plasmid

#208972

Purpose

Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29(+exo)-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA3 (pLP305)

Plasmid

#196992

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.

Depositor

Matthew Maurano

Article

Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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GPX8 rescue PLAK1

Plasmid

#161517

Purpose

Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

glutathione peroxidase 8 (GPX8 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

Plasmid

#105023

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfb (Cobra1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

Plasmid

#105024

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfb (Cobra1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-HA-2xMCP-Ezh2

Plasmid

#126590

Purpose

Expresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-Ezh2 (Ezh2 Synthetic, Mouse)

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Mutation

Promoter

human ubiquitin C promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPICZ:mTREK-1_cryst

Plasmid

#133269

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Use

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Promoter

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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BAM3 X078_pECIA2

Plasmid

#115106

Purpose

Bait vector BAM3 X078_pECIA2 should be used with prey vector BAM3 X078_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G20270 (AT4G27020 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

Plasmid

#225490

Purpose

Neuronal expression vector for generating mTREK-1 (S131C/A286F) + IRES - GFP under the hSyn promoter

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Neuronal expression

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

Plasmid

#224779

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C / S562C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

Plasmid

#224755

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C/C159A/C219A/C365A/C399A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

Plasmid

#224756

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131A/C159A/C219A/C365A/C399A

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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