Addgene

We narrowed to 20,444 results for: crispr

Showing: 4001 - 4020 of 20444 results

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pSB700 lenti gRNA Hygro

Plasmid

#167907

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xFLAG-Blast

Plasmid

#207717

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al bioRxiv

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-3xFLAG

Plasmid

#207684

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al bioRxiv

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Use

Tags

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHFS:SEP-rGluA1 TKIT donor

Plasmid

#169438

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro

Plasmid

#167911

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHFS:SEP-rGluA2 TKIT donor

Plasmid

#169440

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A-1x4

Plasmid

#58768

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-miRFP670nano3

Plasmid

#207680

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

Depositor

Laurence Pelletier

Article

Philip et al bioRxiv

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMV261-Cas12a

Plasmid

#158707

Purpose

expresses codon-optimized FnCpf1

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

FnCpf1

Use

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA3 TKIT donor

Plasmid

#169425

Purpose

Donor DNA for TKIT knockin of SEP to GluA3 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniT:SEP-GluN1 TKIT donor

Plasmid

#169427

Purpose

Donor DNA for TKIT knockin of SEP to NR1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA562

Plasmid

#186237

Purpose

pTet-RfxCas13d crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge