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Plasmid#100095PurposeSNIPer plasmid, N terminal TEV fused to FrbDepositorInsertN-TEV
UseRetroviralTagsFrb and MycExpressionMammalianMutationN terminal domainPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Nesprin-1αKASH
Plasmid#187015PurposeDisruption of LINC ComplexDepositorInsertNesprin-1alphaKASH
UseLentiviralTagsmCherryExpressionMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-C-TEV 219
Plasmid#100096PurposeSNIPer plasmid, C terminal TEV fused to FKBPDepositorInsertC-TEV
UseRetroviralTagsFKBP and MycExpressionMammalianMutationC terminal domainPromoterCMVAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
UseTags6xHis tag and HRV3C cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
UseTagsHRV3C cleavage site and StrepII-tag withExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAID 1.1 N-T2A-Bsr
Plasmid#105985PurposeTo make an auxin inducible degron mutantDepositorInsertT2A-Bsr
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Vrg4-V5-APEX2-URA
Plasmid#207071PurposeExpressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.DepositorInsertVrg4
UseTagsV5-APEX2ExpressionYeastMutationPromoterpVRG4Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEP110E
Plasmid#97396Purposebinary construct of nuclear mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsNucleus targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAID 1.1C-T2A-Bsr
Plasmid#105986PurposeTo make an auxin inducible degron mutantDepositorInsertT2A-Bsr
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)
Plasmid#185802Purposeone plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
P2X4-pHluorin123
Plasmid#52926Purposeexpress P2X4 protein with pHluorin at 123rd positionDepositorInsertmP2X4-pHluorin123
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
PEP109E
Plasmid#97395Purposebinary construct of cytosolic mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC15-P1B3
Plasmid#202856PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15DepositorInsertEGFP/BC15
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC11-P1A11
Plasmid#202852PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11DepositorInsertEGFP/BC11
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrans_9xD3B_Cluc_Norm
Plasmid#68415PurposeCRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.DepositorInsertCypridina Luciferase-2A-Venus YFP
UseLuciferaseTagsExpressionMammalianMutationYFP: M153T,V163A,S175G ("Venus") YFP Va…PromoterminimalCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)
Plasmid#185801Purposeone plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
USP19 sgRNA1
Plasmid#78585Purposedelete USP19 gene in human cellDepositorInsertUSP19 sgRNA1
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
SS-HA-Sun1L-KDEL
Plasmid#187016PurposeDisruption of LINC ComplexDepositorInsertSS-HA-Sun1L-KDEL
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PEP104E
Plasmid#97390Purposebinary construct of mitochondria-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsmitochondria targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJV0010
Plasmid#205132PurposeExpression of 1xPX-GFP in DictyosteliumDepositorInsert1xPX domain of SnxA
UseDictyostelium expressionTagsEGFPExpressionMutationPromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pChimera
Plasmid#61476PurposeVector for conventional cloning that contains AtU6-26 promoter and sgRNA backboneDepositorInsertU6-26:sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-TPC
Plasmid#61478PurposeBinary expression vector with A.th. codon-optimized Cas9 for conventional cloningDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-PA-PKI
Plasmid#65456Purposephotoactivatable PKA inhibitionDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pISH-Tshz1
Plasmid#74360PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.DepositorInsertteashirt zinc finger family member 1 (Tshz1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)
Plasmid#185800Purposeone plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1DepositorInsertcmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-RalGDS
Plasmid#118315PurposeExpression of human RalGDSDepositorInsertRalGDS (RALGDS Human)
UseTagsGFPExpressionMammalianMutationfragment of human RalGDS 203aaPromoterCMVAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS1+2)
Plasmid#69244PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-LBR-EGFP-polyA
Plasmid#59748PurposeExpress LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.DepositorInsertLBR-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_BR29_00091361
Plasmid#121209PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertBR29_00091361
UseGolden gateTagsExpressionMutationMagnaporthe griseaPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMRXIP-3xMyc-STX17FL
Plasmid#89942PurposeExpresses syntaxin17 tagged with Myc in mammalian cellsDepositorInserthuman syntaxin17 (STX17 Human)
UseRetroviralTagsMycExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP112E
Plasmid#97398Purposebinary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsMitochondria targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISH-Barx1
Plasmid#84306PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.DepositorInsertBarx1 (Barx1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
PEP111E
Plasmid#97397Purposebinary construct of plastid-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsPlastid targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
smHA multi-frame tag
Plasmid#128611Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.DepositorInsertsmHA MF tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBORF-DBD
Plasmid#127463PurposeORF filtering plasmid (lambda cI associated plasmid for pAVA)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBORF-AD
Plasmid#127462PurposeORF filtering plasmid (RNAp associated plasmid for pAVA)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV/MASC_(GLuc)_TOP1
Plasmid#68439PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.DepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPDK3181
Plasmid#97403Purposebinary construct of ER-targeted sfCherry1-10 for plant expressionDepositorInsertER-sfCherry1-10
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-USP19
Plasmid#78593PurposeExpress mCitrine-USP19 in mammalian cellsDepositorInsertUSP19 (USP19 Human)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPDK3182
Plasmid#97404Purposebinary construct of ER-targeted sfYFP1-10 for plant expressionDepositorInsertER-sfYFP1-10
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3184
Plasmid#97405Purposebinary construct of cytosolic sfCFP1-10 tag for plant expressionDepositorInsertsfCFP1-10
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP115E
Plasmid#97401Purposebinary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsGolgi targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Apple1
Plasmid#132749PurposeEncodes an iconic representation of an appleDepositorInsertApple1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEP116E
Plasmid#97402Purposebinary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsPlasma membrane targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP108E
Plasmid#97394Purposebinary construct of plasma membrane-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsPlasma membrane targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP106E
Plasmid#97392Purposebinary construct of ER-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only