-
Plasmid#213918PurposeAAV construct expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8mDepositorInsertjGCaMP8m
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
BK Dunlop
Plasmid#25466DepositorInserthuman papovavirus BK Dunlop
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_AXIN2_WT
Plasmid#82099PurposeGateway Donor vector containing AXIN2 , part of the Target Accelerator Plasmid Collection.DepositorInsertAXIN2 (AXIN2 Human)
UseGateway entry vectorTagsExpressionMutationP50SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry
Plasmid#161734PurposeDoxycycline-inducible expression of mStrawberryDepositorInsertmStrawberry
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60228PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianMutationPromoterCBh and U6Available sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G BCL2L11 Neo
Plasmid#140747PurposeInducibly expresses BIM in mammalian cellsDepositorInsertBCL2L11 (BCL2L11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV TRE3GAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SuperClomeleon
Plasmid#203724PurposeAn improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chlorideDepositorInsertCerulean linked topaz fluorescent protein
UseTagsExpressionBacterial and MammalianMutationCerulean, modified Topaz Fluorescent Protein with…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
7TP
Plasmid#24309DepositorInsert7xTcf-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#126217PurposeLabeling of inhibitory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP39
Plasmid#22581DepositorInsertUSP39 (USP39 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR3c-V5/HIS
Plasmid#201108Purposeexpression of human FGFR3 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis WT
Plasmid#213603PurposeExpresses PLA2G15-6xHis in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9_sgAAVS1
Plasmid#199213PurposeAll-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.DepositorInsertAAVS1-gRNA
UseCRISPRTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1ab-Prkd1
Plasmid#139314PurposeDiacylglycerol biosensorDepositorInsertmCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
UseTagsExpressionMammalianMutationT208A, N313DPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG241 FKBP-CFAST10
Plasmid#130814PurposeExpresses FKBP-CFAST10 in mammalian cellsDepositorInsertFKBP-CFAST10
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1083
Plasmid#143922PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFUBP1 (FUBP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
R5 PV
Plasmid#51734PurposeDicistronic vector with PV IRESDepositorInsertPV IRES
UseTagsExpressionMutationPromoterSV40Available sinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xFLAG-V5-ccdB
Plasmid#87064PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-VprBP
Plasmid#133586PurposeExpresses human VprBP wildtype in mammalian cellsDepositorInsertVpr (HIV-1) binding protein (DCAF1 Human)
UseTagsHAExpressionMammalianMutationWildtype human VprBP (Dcaf1)PromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
MED12_pLX307
Plasmid#98350PurposeLentiviral expression of MED12DepositorInsertMED12 (MED12 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR1392QPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-BCR-ABL-p210-FLAG
Plasmid#205619PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorInsertBCR-ABL oncogene, p210 isoform b3a2
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin
Plasmid#60613PurposeEncodes YFP and NLS tagged beta-actinDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQXCINeo-LOXL2-BC
Plasmid#134763Purposea retroviral vector to express lysyl oxidaseL2DepositorInsertLOXL2 (LOXL2 )
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
barII-UT-ABAleon2.1
Plasmid#106981PurposeABAleon2.1 in plant expression vector (Basta resistance)DepositorInsertABAleon2.1
UseTagsExpressionPlantMutationPromoterUBQ10Available sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
GFP-PBRM1
Plasmid#65387PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertPBRM1 (PBRM1 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-TurboID-NES_pLX304
Plasmid#202008Purposeexpresses TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-TurboID-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_PAX7_P2A_Hygro_Barcode
Plasmid#120472PurposeBarcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertPAX7 (PAX7 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
myc-mTOR (1-1482 aa)
Plasmid#21745DepositorInsertmTOR (1-1482) (Mtor Rat)
UseTagsmycExpressionMammalianMutationamino acids 1-1482PromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO1B3_STOP
Plasmid#161366PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO1B3 (SLCO1B3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-FKBP-SAC1deltaTMD
Plasmid#108132PurposeRecruitable cytosolic SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-521)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ING2
Plasmid#109502PurposeMuscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsertactc1b-mKate2-ING2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-FLAG-hphMX4
Plasmid#20752DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158652Purposemaking transgenics expressing a UAS driven eGFP and NTR 2.0DepositorInsert5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF0984
Plasmid#141779PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF5 (KLF5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_GNA13_WT
Plasmid#81963PurposeGateway Donor vector containing GNA13, part of the Target Accelerator Plasmid Collection.DepositorInsertGNA13 (GNA13 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 Y701F
Plasmid#12302DepositorInsertSTAT1 Y701F (STAT1 Human)
UseTagseGFPExpressionMammalianMutationY701FPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF2364
Plasmid#142051PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREB1 (CREB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-IL6
Plasmid#111933PurposeExpresses human interleukin-6 (IL6) fused to GFP in mammalian cellsDepositorInsertInterleukin 6 (IL6 Human)
UseTagsGFPExpressionMammalianMutationCodon optimized for expression in human cellsPromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1707
Plasmid#141868PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBNC2 (BNC2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
EHD2-mEGFP
Plasmid#45932Purposecaveolar protein, confines caveolae to the plasma membraneDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmonomeric EGFP_FrameN1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUW-Luc-mCherry-puro
Plasmid#183685PurposeLentiviral vector expressing luciferase and mCherry.DepositorInsertLuc-E2A-mCherry-T2A-PuroR
UseLentiviralTagsExpressionMutationPromoterHuman Ubiquitin C (UbC) promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-Ago2
Plasmid#74231PurposeGFP-Ago2 lentiviral vectorDepositorInsertAgo2 (AGO2 Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-2PH-PLCd-mKate2
Plasmid#109500PurposeMuscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.DepositorInsertactc1b-2PH-PLCd-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1_IVT
Plasmid#222340PurposePlasmid for wild type Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized wtBxb1
UseTemplate for in vitro transcriptionTagsExpressionMutationPromoterT7 (inactivated)Available sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-WT SpCas9-NLS-6xHis
Plasmid#207373PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertWT SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWS5390-CRISPRai-URA3
Plasmid#182829PurposeSaccharomyces cerevisiae inducible CRISPRai vector - URA3 markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only