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Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NMYC TRF2
Plasmid#16066DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsMycExpressionMutationaa2-500, inserted alanin at aa434PromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE
Plasmid#104488PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9InsertjGCaMP7f
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-hChR2(H134R)-EYFP
Plasmid#26973PurposeAAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
p181 pK7-HDAC1 (GFP)
Plasmid#11054DepositorInsertHDAC1 (Hdac1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmal_c5x_RNAse_Inhib
Plasmid#153314PurposeBacterial expression of an RNase inhibitorDepositorInsertRNAse Inhibitor
UseTagsMaltose Binding ProteinExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)
Plasmid#213029PurposeExpresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W
Plasmid#67981PurposeCas9 activity reporter (control) with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG
Plasmid#105537PurposeAAV expression of Cre from CMV promoterDepositorHas ServiceAAV rh10, AAV1, AAV2, AAV8, and AAV9InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)
Plasmid#192190PurposeHER2 CAR AAV vector PRODH2 KI (pLY080b)DepositorInsertHER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
UseAAV; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH47751
Plasmid#48002DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PRB
Plasmid#89130Purposemammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418DepositorInserthuman progesterone receptor-B (PGR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECJ3
Plasmid#75465PurposeToggle switch plasmid containing lacI-pdt3DepositorInsertlacI-pdt3
UseTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLENTI6.3/AR-GC-E2325
Plasmid#85128Purposeexogenous AR expressionDepositorInsertandrogen receptor (AR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR-Hygro
Plasmid#176063PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertLivePAR
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP16
Plasmid#22595DepositorInsertUSP16 (USP16 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NUP98_HOXD13
Plasmid#205871PurposeExpress mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Brainbow3.2-PGK-PuroTK
Plasmid#159928PurposeBrainbow transgenesisDepositorInsertsNFPnls
mOrange2
EGFP
mKate2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-Neo
Plasmid#128660Purposemammalian expression of EGFP with neomycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK305
Plasmid#66812PurposeIVT vector for Fluc mRNADepositorInsertFirefly luciferase
UseLuciferase; Mrna ivtTagsExpressionMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBudORF1-CH
Plasmid#51290Purposeexpresses the codon optimized ORF1 of L1RP with his tagDepositorInsertL1 ORF1 (L1RE1 Human)
UseTagsHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
wt dynamin 2 pEGFP
Plasmid#34686DepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsEGFPExpressionMammalianMutationD406G, V664A, H692R and Q758RPromoterCMVAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-ATR WT
Plasmid#31611DepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only