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Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
UseTagsHexahistidine tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIOX
Plasmid#51300PurposeE.coli expression vector for His8-SUMO tagged recombinant proteinDepositorTypeEmpty backboneUseTagsHis8 + SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSG058
Plasmid#214261PurposePaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)DepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
UseTags6xHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pRC/CMV
Plasmid#8514DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionMammalianMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMS1088
Plasmid#80159PurposepET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Cys-4xNuG2-Ald
UseTagsHisSUMOExpressionMutationPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS1251
Plasmid#80163PurposepET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Ald-4xNuG2-Ald
UseTagsHisSUMOExpressionMutationPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG059
Plasmid#214262PurposePaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)DepositorInserttLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
UseTags6x His-tag and 6xHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT-eGFP
Plasmid#215168PurposeEncodes the enhanced green fluorescent proteinDepositorInsertWT-eGFP
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pfu-MBP
Plasmid#215156PurposeEncodes a thermostable maltose binding proteinDepositorInsertPfu-MBP
UseTagsExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stag-ObMT
Plasmid#215161PurposeShanbhag lab plasmidsDepositorInsertStag-ObMT
UseTagsS-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBK092
Plasmid#183146PurposepET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expressionDepositorInsertCrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-GST
Plasmid#215169PurposeEncodes the synthetic glutathionine S-transferase with N-terminal His tagDepositorInsertHis-GST
UseTagsHis-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-YCup1
Plasmid#215157PurposeEncodes a thermostable maltose binding protein fused to yeast copper metallothioneinDepositorInsertMBP-YCup1
UseTagsExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only