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Showing: 3981 - 4000 of 20444 results

  2. qTAG-N-Puro-moxGFP

    Plasmid
    #207688
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207688-plasmid-map-sequence-id-413751

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  4. pCas12f1

    Plasmid
    #171182
    Purpose
    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
    Depositor
    Kohsuke Honda
    Article
    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124
    Insert
    Cas12f1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cas9 promoter from Streptococcus pyogenes
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171182-plasmid-map-sequence-id-336407

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  5. pJAT75

    Plasmid
    #204306
    Purpose
    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204306-plasmid-map-sequence-id-406175

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  8. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62888-plasmid-map-sequence-id-105911

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  10. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    62887-plasmid-map-sequence-id-105910

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  12. qTAG-N-Puro-miRFP670nano3

    Plasmid
    #207691
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207691-plasmid-map-sequence-id-413754

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  17. qTAG-C-miniTurbo-Blast

    Plasmid
    #207714
    Purpose
    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207714-plasmid-map-sequence-id-413771

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  19. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61272-plasmid-map-sequence-id-99585

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Showing: 3981 - 4000 of 20444 results