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Showing: 3981 - 4000 of 5232 results

  1. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  2. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  3. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  8. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51084-plasmid-map-sequence-id-172966

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  9. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  14. pLentiN

    Plasmid
    #37444
    Purpose
    Control lentiviral plasmid containing FLAG-HA tags
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    FLAG-HA
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid
    #110568
    Purpose
    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
    Depositor
    Jeffrey Friedman, Alexander Nectow
    Insert
    3xFLAG/HA-tagged anti-GFP VHH
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110568-plasmid-map-sequence-id-213334

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  16. pLV hMyoD-IRES-dsRed

    Plasmid
    #66631
    Purpose
    Co-expresses human MyoD and dsRed
    Depositor
    Charles Gersbach
    Insert
    human MyoD IRES dsRed (MYOD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hUbc
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66631-plasmid-map-sequence-id-114011

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  17. AAV Ef1a-mRuby3-WPRE

    Plasmid
    #135427
    Purpose
    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135427-plasmid-map-sequence-id-271155

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Showing: 3981 - 4000 of 5232 results