-
Plasmid#162282PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pEPQD0KN0024
Plasmid#162281PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0539
Plasmid#162306PurposeLevel 0 CDS part for MoClo assembly, AATG-TTCGDepositorInsertCDS, TEV protease (S219V), no stop codon
UseSynthetic BiologyTagsExpressionMutationS219V, codon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPMY0SP0002
Plasmid#162304PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, 6xHis tag-[HRV 3C cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0551
Plasmid#162303PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0547
Plasmid#162298PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, TrxA-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0546
Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0544
Plasmid#162295PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[Factor Xa cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0543
Plasmid#162294PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0541
Plasmid#162292PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[thrombin cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KinK
Plasmid#139596Purposeexpresses KinK in E. coliDepositorInsertkinK
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KinL
Plasmid#139595Purposeexpresses KinL in E. coliDepositorInsertkinL
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KinM
Plasmid#139594Purposeexpresses KinM in E. coliDepositorInsertkinM
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KinN
Plasmid#139593Purposeexpresses KinN in E. coliDepositorInsertkinN
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR_R1
Plasmid#124209PurposegRNA/Cas9 expression for 2nd editing using pSLHDR01, 02DepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB325
Plasmid#113669Purposeexpresses Ulp1_R1 in Escherichia coliDepositorInsertUlp1_R1
UseTags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc PSM1
Plasmid#106328PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc phosphorylation site mutant SGSSEEKQNAVSSEET…PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc PSM3
Plasmid#106327PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc phosphorylation site mutant SGSSEEKQNAVSSEET…PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc PSM2
Plasmid#106326PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc phosphorylation site mutant SGSSEEKQNAVSSEET…PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M1
Plasmid#106321PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc mutated splice site EEKQ-->AAAAPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only