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Plasmid#198890PurposeExpress AID(1-188) BE3-based base editorDepositorInsertActication induced deaminase (AICDA Human)
UseTagsNLSExpressionMammalianMutationDeletion of amino acids 189-198PromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4-4xmut-dsRNAres_R
Plasmid#147425PurposeInsect Expression of DmCCR4-4xmut-dsRNAresDepositorInsertDmCCR4-4xmut-dsRNAres (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_1-166-L42EI44E-dsRNAres_R
Plasmid#147426PurposeInsect Expression of DmCCR4_1-166-L42EI44E-dsRNAresDepositorInsertDmCCR4_1-166-L42EI44E-dsRNAres (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4-L42EI44E-dsRNAres_R
Plasmid#147429PurposeInsect Expression of DmCCR4-L42EI44E-dsRNAresDepositorInsertDmCCR4-L42EI44E-dsRNAres (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2 gRNA resistant
Plasmid#163408PurposeExpresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationgRNA-targeting sequences are substituted with nuc…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase gRNA resistant
Plasmid#163411PurposeExpresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe kinase domain (202-500 aa) is deleted. gRNA-t…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g3 (BB24)
Plasmid#139459PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1_cryst
Plasmid#133271PurposeX. laevis expression vector. It will generate the TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
UseOocyte expressionTagsExpressionMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…PromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W
Plasmid#105036PurposeLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFPDepositorInsertPou5f1 (Pou5f1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W
Plasmid#105035PurposeLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFPDepositorInsertPou5f1 (Pou5f1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W
Plasmid#105017PurposeLentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFPDepositorInsertKcmf1 (Kcmf1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; K141E
Plasmid#89865PurposeExpresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationmutated lysine 141 to glutamic acidPromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO-BROX 1-411; L212D
Plasmid#89864PurposeExpresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationmutated leucine 212 to aspartic acidPromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRKACG gRNA (BRDN0001148597)
Plasmid#77364Purpose3rd generation lentiviral gRNA plasmid targeting human PRKACGDepositorInsertPRKACG (PRKACG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRKACG gRNA (BRDN0001148941)
Plasmid#77365Purpose3rd generation lentiviral gRNA plasmid targeting human PRKACGDepositorInsertPRKACG (PRKACG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRKACG gRNA (BRDN0001149492)
Plasmid#77366Purpose3rd generation lentiviral gRNA plasmid targeting human PRKACGDepositorInsertPRKACG (PRKACG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRKD1 gRNA (BRDN0001162316)
Plasmid#77229Purpose3rd generation lentiviral gRNA plasmid targeting human PRKD1DepositorInsertPRKD1 (PRKD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN1 gRNA (BRDN0001149250)
Plasmid#76275Purpose3rd generation lentiviral gRNA plasmid targeting human PKN1DepositorInsertPKN1 (PKN1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only