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Plasmid#133770Purposeexpress 6Xhis- SUMO 1 proteinDepositorInsertSUMO 1 (SUMO1 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-Blast
Plasmid#109334PurposepInducer20 with added blasticidin resistanceDepositorTypeEmpty backboneUseLentiviral; Inducible expressionTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239D
Plasmid#22220DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDM4
Plasmid#49796PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-Halo
Plasmid#193328Purposemammalian transient expression - expression of Lifeact-HaloDepositorInsertLifeact
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-IDH1
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorInsertisocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF1A
Plasmid#172206PurposeExpresses KIF1A tagged with eGFP in mammalian cellsDepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg1
Plasmid#127158PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotypeDepositorInsertIghg1 (Ighg1 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-RBM3
Plasmid#87860PurposeExpresses human RBM3 coupled to GFPDepositorInsertRNA binding motif protein 3 (RBM3 Human)
UseTagsGFPExpressionMammalianMutationNAPromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRU1099
Plasmid#14464DepositorInsertunstable gfp-mut3.1 AAV
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNLS-iRFP713
Plasmid#45468DepositorInsertiRFP713
UseTagsnuclear localization signalExpressionMammalianMutationT2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
PX458-AAVS1
Plasmid#113194PurposeEncodes gRNA for human AAVS1DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS2-blast
Plasmid#158458PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)
Plasmid#208957PurposeA variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
nNOS/NOS1 scFv [L121/133]
Plasmid#206759PurposeMammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/133 scFv.DepositorInsertnNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-dADAM17 (#1)
Plasmid#177259PurposeA knockout vector for the dog Adam17DepositorInsertA gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mPlum-N1
Plasmid#54629PurposeLocalization: N1 Cloning Vector, Excitation: 590, Emission: 649DepositorTypeEmpty backboneUseTagsmPlumExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
Plasmid#140002PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFH2.2_mCherry-MS2x12
Plasmid#205521PurposeExpress mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-FLPo-T2A-GFP
Plasmid#161766Purposeexpress FLPo and GFP under the control of human synapsin promoterDepositorInsertsFLPo
T2AEGFP
UseAAVTagsExpressionMammalianMutationPromoterhSynapsin1Available sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_eGFP
Plasmid#221463PurposeAAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFLP3
Plasmid#64946PurposeFlp recombinase expression vectorDepositorInsertFlp recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHCMV_FLUBV_HA
Plasmid#207274PurposeMammalian expression of Influenza B virus HA proteinDepositorInsertFLUBV_HA (HA Influenza B virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCG_batCoV RaTG13 N C-V5-tag
Plasmid#179987PurposeMammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.DepositorInsertRaTG13 N
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer-YAP1-S127A/S397A
Plasmid#213585PurposeDoxycycline inducible expression of YAP1-S127A/S397A cDNA in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationS127A, S397APromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2890
Plasmid#142168PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR' CMV GFP
Plasmid#14858DepositorInsertCMV-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 N C-V5-tag
Plasmid#179968PurposeMammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 N (N Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGNSTM glycosylation motif, HA, Lamp2 signal pepti…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 C-V5-tag ORF3a
Plasmid#179969PurposeMammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 ORF3a (ORF3a Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5E-slc1a3b
Plasmid#170205PurposeGateway 5’ entry vector with the slc1a3b promoterDepositorInsertslc1a3b (slc1a3b )
UseUnspecified; ZebrafishTagsExpressionMutationPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean N1
Plasmid#27795DepositorInsertmCerulean N1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
VN-alpha Synuclein
Plasmid#89470Purposeexpresses human alpha Synuclein fused to the N-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only