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Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a
Plasmid#205310PurposeProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoterDepositorInsertWHaloCaMP1a
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX NPC1-FLAG-TiD
Plasmid#199748PurposeLentiviral construct to stably express NPC1 TurboIDDepositorInsertNPC1 (NPC1 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-hCOL1A1
Plasmid#140112PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmCeruleanExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-OVOL2-HA
Plasmid#184737PurposeDoxycycline-inducible lentiviral expression of OVOL2DepositorInsertovo like zinc finger 2 (OVOL2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterTet ONAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1
Plasmid#108098PurposeLentiviral expression plasmid of sgRNA with GFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_EBOV
Plasmid#103050PurposeEncodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-ZscHCVP-TEVPZ
Plasmid#116094PurposeExpresses a combination of proteasesDepositorInsertZscHCVP-TEVPZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hIFITM3
Plasmid#58397PurposeExpressed human IFITM3 with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS1951
Plasmid#105868PurposeHigh copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insertDepositorUseTagsExpressionMutationPromoterHsp68 MiniPromoterAvailable sinceFeb. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPSU10-30-50-100
Plasmid#173838PurposeCoexpresses 10, 30, 50 and 100 kD Penn State ladder proteinsDepositorInsertSTRHSTPAB
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
UseTagsExpressionBacterialMutationPromoterArabinose-inducible pBADAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
OpenCRISPR-1 sgRNA-16nt stem
Plasmid#221566PurposeExpress OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.DepositorInsertOpenCRISPR-1 sgRNA 16nt stem loop
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
OpenCRISPR-1 sgRNA-12nt stem
Plasmid#221567PurposeExpress OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.DepositorInsertOpenCRISPR-1 sgRNA 12nt stem loop
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PSMA4_GFP_Cterm
Plasmid#221514PurposeFluorescently tagged Psma4-GFPDepositorInsertPSMA4 (PSMA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK33-V5-TurboID-GW
Plasmid#196282PurposeGateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell linesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26
Plasmid#201685PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 geneDepositorInsertNme2-ABE8e-i1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26
Plasmid#201686PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 geneDepositorInsertNme2-ABE8e-i1 (V106W)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only