Addgene

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Rb-379-792-pGex2TK

Plasmid

#21748

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-792 of wt Rb …

Promoter

Available since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pEHIShqprt

Plasmid

#92166

Purpose

Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

Quinolinate Phosphoribosyltransferase (QPRT Human)

Use

Tags

TEV protease -cleavable N-terminal 6His

Expression

Bacterial

Mutation

changed Asp 2 to Asn to create BspHI site

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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432 pSG5L HA RB 567L

Plasmid

#10725

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB 567L (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

567L

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pWzyE-GST-distrepII

Plasmid

#196666

Purpose

Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

GST and TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWzyE-distrepII

Plasmid

#196667

Purpose

Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-RB1-shRNA63

Plasmid

#25641

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 63 (RB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-SSeCKs T751A

Plasmid

#107142

Purpose

full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs T751A (Akap12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed threonine 751 to alanine

Promoter

gag

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + T373

Plasmid

#58912

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T373 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
416 pSG5L HA RB del22

Plasmid

#10721

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del22 (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of exon 22 (aa738-775)

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
409 pSG5L HA RB (379-928)

Plasmid

#10734

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (379-928) (RB1 Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 379-928

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S780

Plasmid

#58915

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S780 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
496 pSG5L HA RB del651 (NAAIRS)

Plasmid

#10726

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del651 (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

residues 651 to 656 were replaced by NAAIRS, a fl…

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
500 pSG5L HA RB del685 (NAAIRS)

Plasmid

#10729

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del685 (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

residues 685 to 690 were replaced by NAAIRS, a fl…

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S811

Plasmid

#58919

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S811 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S788

Plasmid

#58916

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S788 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T356

Plasmid

#58911

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T356 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S807

Plasmid

#58918

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S807 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T826

Plasmid

#58921

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T826 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only