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Plasmid#141091PurposepH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEPDepositorInsertAntares-T2A-puromycin-IRESv24-eGFP-Nanoluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgAAVS1
Plasmid#187818PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMVSP6-nEGFP-SV40-PURO
Plasmid#138364PurposeLentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selectionDepositorInsertnuclear EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
INS-2A-luciferase-2A-tdT
Plasmid#159348PurposeHuman INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.DepositorInsertLuciferase-2A-tdTomato
UseIns locus knockin donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA_118
Plasmid#133459PurposeU6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrRDepositorInsertcontrol guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-CMV:GFP
Plasmid#68485PurposeRetrovirus expressing GFP driven by CMV promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterCMV and LTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P07
Plasmid#124788PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLucIPZ
Plasmid#53222PurposeConstitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR1068
Plasmid#111092PurposeBFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationBlpI site disruptedPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-EGFP-TM-IRES-Pac
Plasmid#183603PurposeMammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHuman PDGFRB transmembrane domain and Mouse IgGK …ExpressionMammalianMutationPromoterCAGAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-T3G-ERP2
Plasmid#175188PurposeAll-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2
Plasmid#175497PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-PURO
Plasmid#58246PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selectionDepositorInsertPuro(R)
UseLentiviral and RNAi; Gateway destionation vectorTagsTetR-NLS-T2AExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPBT-peRNA_GG-Puro
Plasmid#173220PurposepiggyBac transposon containing hU6 promotor and pegRNA cloning siteDepositorInsertspegRNA cloning cassette
PuroR
UsePiggybacTagsExpressionMammalianMutationPromoterEF1-a and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-mGreenLantern/EGFP
Plasmid#179913PurposeAll in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.DepositorInsertmGreenLantern/EGFP sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_212
Plasmid#133457PurposeU6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistanceDepositorInsertcontrol guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P12
Plasmid#120023PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarletDepositorInsertmScarlet
UsePcr templateTagsmScarletExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P09
Plasmid#120020PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenusDepositorInsertmVenus
UsePcr templateTagsmVenusExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only