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Plasmid#224756PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131A/C159A/C219A/C365A/C399APromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS
Plasmid#140193PurposeChromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.DepositorInsertsdLbCas12a-VPR
Bar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
UseCRISPR and Synthetic Biology; Fungal genome integ…TagsVPR (VP64-p65-Rta), NLSExpressionMutationD832A DNAse deactivatedPromoterPgpdA and PtrpCAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS
Plasmid#140196PurposeChromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.DepositorInsertsdSpCas9-VPR
Bar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
UseCRISPR and Synthetic Biology; Fungal genome integ…TagsVPR (VP64-p65-Rta) , NLSExpressionMutationPromoterPgpdA and PtrpCAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert
Plasmid#111153Purposeknock in bait for Axolotl Pax7 locusDepositorInsertGAP43-Cherry-T2a-mERt-Cre-Ert
UseCre/LoxTagsExpressionMutationwtPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119067PurposeDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertNLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119065PurposeDonor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertHA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH10
Plasmid#188783PurposeCarries a NAT marker and C. albicans RBT5 promoter in a pGEM-T backboneDepositorInsertRBT5 promoter
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGMβ1
Plasmid#216202PurposeChromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.DepositorTypeEmpty backboneUseShuttle vector, conjugal plasmid, theta type rep…TagsExpressionBacterialMutationPromoterlac promoter in pGEM-Teasy, Promoters in pAMbeta1…Available sinceJan. 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
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XP4 Xwnt-5A
Plasmid#16987DepositorInsertXwnt-5A (wnt5a.L Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLEW100v5-trunc
Plasmid#33346DepositorInsertLuciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
UseTrypanosoma brucei expression vectorTagsExpressionMutation21bp of wt luciferase deletedPromoterAvailable sinceDec. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
SF1
Plasmid#123001Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sab4
Plasmid#123008Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
R018
Plasmid#123005Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CVB
Plasmid#123003Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AP
Plasmid#123016Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FD
Plasmid#123010Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
RS
Plasmid#123012Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cr
Plasmid#123014Purposecontains the chaperonin-60 universal target from phytoplasma baceria; used as a control for development of molecular diagnostic assaysDepositorInsertchaperonin-60 universal target
UseMolecular diagnostic assay controlTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only