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SPIC_pet28a

Plasmid

#131654

Purpose

Bacterial expression of ETS gene SPIC

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPIC (SPIC Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_STS

Plasmid

#139799

Purpose

expresses biosynthetic enzyme STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS (STS Mustard Weed, vitis vinifera)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-C

Plasmid

#156471

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-C (ORF1ab Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_STS:P4S

Plasmid

#139797

Purpose

expression of biosynthetic enzyme STS in fusion with CC segment P4S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS:P4S

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S

Plasmid

#139796

Purpose

expression of biosynthetic enzyme 4CL in fusion with CC segment P3S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

4CL:P3S

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:STS (DF)

Plasmid

#139791

Purpose

direct fusion of biosynthetic enzymes 4CL and STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

direct fusion of 4CL and STS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d_SNORD78_probe

Plasmid

#73072

Purpose

antisense probe for detection of human SNORD78 (C/D box snoRNA U78)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD78 (SNORD78 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Use

Tags

His6 and S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b_PfSSB_W166C

Plasmid

#103001

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB (C93A,W166C), used for obtaining a fluorescent adduct at the cysteine

Depositor

Martin Webb

Article

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB_W166C

Use

Tags

Expression

Bacterial

Mutation

C93A, W166C

Promoter

T7

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T+1X

Plasmid

#108188

Purpose

PCNA1-PdX fusion protein

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1-PdX fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

C73S/C85S (PdX)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7P

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET24a_UDP_Xase_E162

Plasmid

#73160

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Use

Tags

Expression

Bacterial

Mutation

change glutamate 162 to alanine

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pET11_DHNTPase

Plasmid

#73154

Purpose

Expresses in E. coli, E. coli Dihydroneopterin triphosphate pyrophosphatase

Depositor

Sandra Gabelli, Maurice Bessman

Article

Gabelli et al Structure. 2007 Aug;15(8):1014-22.

Insert

ORF17_DHNTPase_NUD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2016

Availability

Academic Institutions and Nonprofits only