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Plasmid#99673PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-p65(101-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAS1
Plasmid#58252PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locusDepositorInsertU6.gRNAS1 cassette
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH
Plasmid#56659PurposeDual-expression vector for P. tricornutumDepositorInsertKanR
UseSynthetic Biology; Diatom expressionTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGATOR
Plasmid#64102PurposeTet inducible, Gateway adapted ROSA26 targeting VectorDepositorInsertrTTA (Gt(ROSA)26Sor Synthetic)
UseMouse Targeting; Tet inducibleTagsExpressionMammalianMutationPromoterROSA26 endogenousAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDY-HA1-HA2-neo
Plasmid#109123PurposeFR cassette provider to introduce FR cassette to a BAC by recombineeringDepositorInsertsHA1
HA2
UseTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNA polymerase beta prime subunit FLAG
Plasmid#102337PurposeTargeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.DepositorInsertRNAP Beta Prime Subunit with C-terminal FLAG TAG
UseTags3x FLAG with 3X GSGS linkerExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJin-290
Plasmid#125718PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-CDepositorInsertPCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS5
Plasmid#55750PurposeProduces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationResidues 59-264 of KanRPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInsertermC (ermM Staphylococcus epidermis)
UseTags6xHisExpressionBacterialMutationPromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK300
Plasmid#87766Purposeempty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertrmtB (RmtB Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP2
Plasmid#168023PurposeExpresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cellsDepositorInserteCFP-VAMP2(T27-R86)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 vph
Plasmid#112893PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvph
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 vatD
Plasmid#112885PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvatD
UseTagsExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(150-261)-RTA(125-190)
Plasmid#99674PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-P65(151-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL6 SMR1747
Plasmid#28029DepositorInsertMutS (mutS E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(9)-Ia
Plasmid#112882PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertaph(9)-Ia
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEND-321_Cacnes_ori
Plasmid#225603PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only