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Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
UseTagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
UseTagsGFP, S-tag, and TEV siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
Activin A (S2-2)
Plasmid#184621Purposefor transfection into S2 cells and expression of Activin A proteinDepositorInsertINHBA (INHBA Human)
UseTags8x His - streptavidin binding peptideExpressionInsectMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL2299
Plasmid#186332PurposeThis plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.DepositorInsertvirulence genes from Agrobacterium tumefaciens pTiBo542
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
MGAT1-GFP
Plasmid#163647PurposeExpresses GFP tagged MGAT1 in mammalian cellsDepositorInsertalpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1[D157N]
Plasmid#27100DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGFP and S-tagExpressionMammalianMutationD157NPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-N-dTAG_OGT
Plasmid#155196PurposeN-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT constructDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianMutationlacking N-terminal MetPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SulR
Plasmid#186425Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoterDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SBP-ST*-GFP
Plasmid#163668PurposeExpresses SBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
UseTagsGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationThis chimera contains the first 113 amino acids o…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK OGT (Neo)
Plasmid#154289PurposeLentiviral vector encoding full length wildtype human OGTDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLAG W96A-GLTP
Plasmid#170741PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertGLTP (GLTP Human)
UseTagsFLAGExpressionMammalianMutationW96APromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG CPTP
Plasmid#170742PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorInsertCPTP (CPTP Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pONSY-mCherry
Plasmid#111874PurposeThis plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertmCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVENUS-N1-FNTA
Plasmid#86859PurposeTo express FNTA with a Venus tag at C terminus.DepositorInsertFNTA (FNTA Human)
UseTagsVenusExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Venus
Plasmid#111875PurposeThis plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertVenus
UseCapsaspora owczarzakiTagsVenusExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti.hUbC.H2B-CeruleanFP-2A-Dendra2FP.W
Plasmid#126522PurposeLentivector that expresses H2B-Cerulean and Dendra2 fluorescent proteins from an internal hUbC promoter.DepositorInsertH2B-CeruleanFP-2A-Dendra2FP
UseLentiviralTagsExpressionMutationPromoterhUbCAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
Plasmid#172317PurposeCRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-GlcisPT
Plasmid#203172PurposeYeast expression vector for Giardia cis-prenyltransferaseDepositorInsertUPPS
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only