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pCMV-P-RAN-RFP4

Plasmid

#106410

Purpose

P-RAN-RFP4 (RANbody RFP4::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP4

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP2

Plasmid

#106409

Purpose

P-RAN-RFP2 (RANbody RFP2::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP2

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EV

Plasmid

#202491

Purpose

pCOLA-Gent Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-EV

Plasmid

#202466

Purpose

Empty vector of pSMART-HCKan

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina N et et al Nat Commun 15, 7788 (2024)

Insert

GAL4-sfGFP(1-157)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(158-238)

Plasmid

#220744

Purpose

GAL4-sfGFP(158-238) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina N et et al Nat Commun 15, 7788 (2024)

Insert

GAL4-sfGFP(158-238)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-NIR-FbLAG30

Plasmid

#220746

Purpose

GAL4-NIR-FbLAG30 construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina N et et al Nat Commun 15, 7788 (2024)

Insert

GAL4-NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NIR-FbLAG30

Plasmid

#220748

Purpose

NIR-FbLAG30 expression in bacteria.

Depositor

Vladislav Verkhusha

Article

Barykina N et et al Nat Commun 15, 7788 (2024)

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Tags

Expression

Bacterial

Mutation

For bacterial cytoplasmic expression, the pelB si…

Promoter

T7

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG16

Plasmid

#220749

Purpose

NIR-FbLAG16 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina N et et al Nat Commun 15, 7788 (2024)

Insert

NIR-FbLAG16

Use

AAV and Affinity Reagent/ Antibody

Tags

Expression

Mutation

Promoter

CAG

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG30

Plasmid

#220750

Purpose

NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina N et et al Nat Commun 15, 7788 (2024)

Insert

NIR-FbLAG30

Use

AAV and Affinity Reagent/ Antibody

Tags

Expression

Mutation

Promoter

CAG

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTO-Gbp6-TM-Che

Plasmid

#180022

Purpose

Membrane-bound anti-GFP nanobody conjugated to mCherry

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Gpb6 nanobody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Article

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Use

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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2xMARS-nGFP

Plasmid

#205235

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

Depositor

Jeremy Baskin

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pIREShygro HA-mVenus-mAID

Plasmid

#117714

Purpose

Expression of HA-tagged mVenus fused to mAID

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mVenus-mAID

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-2xMARS-nGFP-puro

Plasmid

#205238

Purpose

Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

Depositor

Jeremy Baskin

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Lentiviral

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-NS

Plasmid

#108885

Purpose

Multisite gateway vector for 5' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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