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pAW8_6XFLAG

Plasmid

#110235

Purpose

Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE_puro_ZFP64_N_3XFLAG

Plasmid

#118693

Purpose

Express 3xflag tagged ZFP64 for flag ChIP experiments

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/B0,+

Plasmid

#112798

Purpose

Expression of ATB0,+

Depositor

Katarzyna Nalęcz

Article

Samluk et al Biochem Biophys Res Commun. 2012 May 25;

Insert

B(0,+)/SLC6A14 (Slc6a14 Rat)

Use

Tags

3 x Flag

Expression

Mammalian

Mutation

L350S

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_YFP

Plasmid

#45278

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

YFP

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pLKO_Ollas.C.FLAG_NGFR

Plasmid

#158311

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

Ollas.C.FLAG

Expression

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO_FLAG.VSVg.C_NGFR

Plasmid

#158260

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

FLAG.VSVg.C

Expression

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_SOX2

Plasmid

#45309

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MCOPS3 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_SLC7A14

Plasmid

#45261

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SLC7A14 (SLC7A14 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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OccludinMut_Flag

Plasmid

#86043

Purpose

Full length Occludin with mutation of PPPY domain at AA9-12 to PAAY

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

OccludinMut (OCLN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

PPPY domain at AA9-12 to PAAY; I52T and Q365P

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_K42R_FLAG

Plasmid

#24486

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42Arg

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRC2_3xflag_NRF2(RR)

Plasmid

#136522

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Entry vector for gateway cloning

Tags

3x FLAG

Expression

Mutation

This plasmid is developed by mutating 3 base pair…

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCHFLAGGR

Plasmid

#1279

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GR (Nr3c1 Rat)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_BFP

Plasmid

#45302

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1mLCAD_8KR_FLAG

Plasmid

#24485

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV_FLAG_hSF3B1

Plasmid

#176590

Purpose

Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

FLAG3

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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