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Plasmid#169436PurposeDonor DNA for TKIT knockin of SEP to Gabrb2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF0185 Cp36_attP-mCherry donor
Plasmid#193468PurposePromoterless mcherry donor for Cp36DepositorInsertCp36_attP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1 Clover-LMNA Donor
Plasmid#122508PurposeHomology repair template for in frame (first exon) clover knock-in of human LMNA geneDepositorInsertHomology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
UseHomology repair template plasmid (donor plasmid)TagsCloverExpressionMutationPromoterAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
ires-eGFP(+HAs) donor-1
Plasmid#87865PurposeExpress eGFP reporter for HDR-induced knock-inDepositorInserteGFP
UseHomologous recombination for gapdh lociTagsExpressionMutationPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 R2pH-LAMP1-3xFLAG
Plasmid#157941Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAG, mCherry, and pHluorinExpressionMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 1
Plasmid#22209DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF0
Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF2
Plasmid#200379PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ctcf-Halo-mAID donor
Plasmid#113103PurposeDonor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequenceDepositorInsertc terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN2A TKIT donor
Plasmid#169429PurposeDonor DNA for TKIT knockin of SEP to NR2A in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-humanCTCF-miniAID-mClover3
Plasmid#136883Purposeknockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCFDepositorInsertCTCF-knockin-homology arm-miniAID-mClover3
UseTagsminiAID-mClover3ExpressionMammalianMutationPromoterno promoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAX6 CKO 2/7 Donor plasmid
Plasmid#68467Purposedonor plasmid for targeting PAX6 locusDepositorInsertFRT flanked exon4 of PAX6 gene (PAX6 Human)
UseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PuroR-P2A-DD-TCOF1-donor
Plasmid#60747PurposeDonor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorTagsExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
H517 SONIC HRASV12 donor
Plasmid#138177PurposeCRISPR SONIC: HRAS G12V donor plasmidDepositorInsertHRASV12 (HRAS Human)
UseNhej donorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI ezh2 donor vector
Plasmid#65620PurposeDonor vector to generate Degron-KI DD-EZH2DepositorInsertEZH2 left and right HR arms
UseTagsExpressionMutationPromoterAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN2B TKIT donor
Plasmid#169431PurposeDonor DNA for TKIT knockin of SEP to NR2B in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only