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Plasmid#13371PurposeRetroviral expression of mouse beta-catenin S33Y mutantDepositorInsertcatenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)
UseRetroviralTagsExpressionMammalianMutationSerine 33 to Tyrosine and Leucine 388 to ProlinePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003
Plasmid#133566PurposeExpresses EGFP-Talin head-SpyCatcher003 in mammalian cellsDepositorInsertEGFP-Talin head-SpyCatcher003
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.2279
Plasmid#19762DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin/α-catenin fusion-pcDNA3
Plasmid#45771DepositorUseTagsExpressionMammalianMutationHuman E-cadherin (aa 1-801) fused to Xenopus α-ca…PromoterCMVAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXL003-ishRNA-beta-catenin-2
Plasmid#36299DepositorInsertshRNA of beta-catenin-2 (CTNNB1 Human)
UseLentiviral and RNAiTagsIRES-RFP-PuroExpressionMammalianMutationPromoterTetO-H1Available sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF019 MBP-β-catenin-His
Plasmid#154070PurposeExpresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCTNNB1 (CTNNB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
human gamma-catenin pcDNA3
Plasmid#16827DepositorInsertgamma-catenin (JUP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-N90-beta-catenin
Plasmid#31787DepositorInsertbeta-catenin (CTNNB1 Human)
UseEntry vectorTagsMycExpressionMutationDeletion of aa 1-90- Constitutively ActivePromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mTet2 catalytic domain HxD
Plasmid#79611PurposeExpression of catalytically inactive mouse Tet2DepositorInsertTet2 (Tet2 Mouse)
UseLentiviralTagsMycExpressionMutationMutant Tet2 with H1302Y, D1304A substitutions in …PromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107
Plasmid#64615Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SpyCatcher-ELP-GFP
Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
UseTags6x His Tag, GFP, and TEV TagExpressionBacterialMutationPromoterT5 promoter/lac operator elementAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107-V5
Plasmid#64616Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsV5ExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmcherry α-catenin M319G/R326E
Plasmid#178648Purposeexpress α-catenin with M319G/R326E mutationDepositorInsertα-catenin (CTNNA1 Human)
UseTagsmcherryExpressionMammalianMutationM319G/R326EPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP6 (catalytic domain)
Plasmid#16359DepositorInserthuman SENP6 catalytic domain (SENP6 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 628 to 1112 catalytic domain of human …PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1 N-terminal truncation
Plasmid#22404PurposeExpresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2–mPKCε-CAT-HA
Plasmid#190483PurposeExpresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAXDepositorInsertmCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)
UseTagsFused to CRY2, HA, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only